In Vitro- and In Vivo-Induced Transgene Expression in Human Embryonic Stem Cells and Derivatives
Article first published online: 21 NOV 2007
Copyright © 2008 AlphaMed Press
Volume 26, Issue 2, pages 525–533, February 2008
How to Cite
Xia, X., Ayala, M., Thiede, B. R. and Zhang, S.-C. (2008), In Vitro- and In Vivo-Induced Transgene Expression in Human Embryonic Stem Cells and Derivatives. STEM CELLS, 26: 525–533. doi: 10.1634/stemcells.2007-0710
- Issue published online: 2 JAN 2009
- Article first published online: 21 NOV 2007
- Manuscript Accepted: 13 NOV 2007
- Manuscript Received: 26 AUG 2007
- Human embryonic stem cell;
- Gene modification;
- Gene delivery systems in vivo or in vitro;
- Inducible gene expression
The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.
Disclosure of potential conflicts of interest is found at the end of this article.