Intra-Bone Marrow Injection of Donor Bone Marrow Cells Suspended in Collagen Gel Retains Injected Cells in Bone Marrow, Resulting in Rapid Hemopoietic Recovery in Mice
Article first published online: 3 JUL 2008
Copyright © 2008 AlphaMed Press
Volume 26, Issue 9, pages 2211–2216, September 2008
How to Cite
Shi, M., Adachi, Y., Shigematsu, A., Koike-Kiriyama, N., Feng, W., Yanai, S., Yunze, C., Lian, Z.-X., Li, J. and Ikehara, S. (2008), Intra-Bone Marrow Injection of Donor Bone Marrow Cells Suspended in Collagen Gel Retains Injected Cells in Bone Marrow, Resulting in Rapid Hemopoietic Recovery in Mice. STEM CELLS, 26: 2211–2216. doi: 10.1634/stemcells.2008-0035
- Issue published online: 2 JAN 2009
- Article first published online: 3 JUL 2008
- Manuscript Accepted: 24 MAY 2008
- Manuscript Received: 12 JAN 2008
- Intra-bone marrow bone marrow transplantation;
- Collagen gel;
- Colony-forming units of spleen;
- Enhanced green fluorescent protein
We have recently developed an innovative bone marrow transplantation (BMT) method, intra-bone marrow (IBM)-BMT, in which donor bone marrow cells (BMCs) are injected directly into the recipient bone marrow (BM), resulting in the rapid recovery of donor hemopoiesis and permitting a reduction in radiation doses as a pretreatment for BMT. However, even with this IBM injection, some of the injected BMCs were found to enter into circulation. Therefore, we attempted to modify the method to allow the efficient retention of injected BMCs in the donor BM. The BMCs of enhanced green fluorescent protein transgenic mice (C57BL/6 background) were suspended in collagen gel (CG) or phosphate-buffered saline (PBS), and these cells were then injected into the BM of irradiated C57BL/6 mice. The numbers of retained donor cells in the injected BM, the day 12 colony-forming units of spleen (CFU-S) counts, and the reconstitution of donor cells after IBM-BMT were compared between the CG and PBS groups. The number of transplanted cells detected in the injected BM in the CG group was significantly higher than that in the PBS group. We next carried out CFU-S assays. The spleens of mice in the CG group showed heavier spleen weight and considerably higher CFU-S counts than in the PBS group. Excellent reconstitution of donor hemopoietic cells in the CG group was observed in the long term (>100 days). These results suggest that the IBM injection of BMCs suspended in CG is superior to the injection of BMCs suspended in PBS.
Disclosure of potential conflicts of interest is found at the end of this article.