Mesenchymal Stem Cells Alter Migratory Property of T and Dendritic Cells to Delay the Development of Murine Lethal Acute Graft-Versus-Host Disease
Article first published online: 17 JUL 2008
Copyright © 2008 AlphaMed Press
Volume 26, Issue 10, pages 2531–2541, October 2008
How to Cite
Li, H., Guo, Z., Jiang, X., Zhu, H., Li, X. and Mao, N. (2008), Mesenchymal Stem Cells Alter Migratory Property of T and Dendritic Cells to Delay the Development of Murine Lethal Acute Graft-Versus-Host Disease. STEM CELLS, 26: 2531–2541. doi: 10.1634/stemcells.2008-0146
- Issue published online: 9 JAN 2009
- Article first published online: 17 JUL 2008
- Manuscript Accepted: 1 JUL 2008
- Manuscript Received: 14 FEB 2008
- Mesenchymal stem cell;
- T lymphocyte;
- Dendritic cells;
- Acute graft-versus-host disease
Due to the potent immunoregulatory capacity, mesenchymal stem cells (MSCs) have been used in clinical trials to treat acute graft-versus-host disease (aGvHD), although the detailed in vivo mechanisms remain elusive. In a murine lethal aGvHD model, MSCs delayed the development of the disease. Interestingly, we found that MSC infusion increased the number of T lymphocytes in the secondary lymphoid organs (SLOs). Since the expression of CD62L and CCR7 is prerequisite for lymphocyte migration into SLOs, the in vitro experiments revealed that in the presence of MSCs, T lymphocytes (including CD4+CD25+ regulatory T cells) preferred to take the naive-like phenotype (CD62L+/CCR7+) in mixed lymphocyte reaction and maintained the migratory activity elicited by secondary lymphoid tissue chemokine (SLC). Dendritic cells (DCs) are the initiator of immune response. CCR7 expression is pivotal for their maturation and migration into SLOs. However, CCR7 expression and SLC-driven migratory activity of DCs were remarkably suppressed by MSC coculture. The processes above were realized mainly through secretory mechanism. Consistently, MSC infusion maintained T lymphocytes to take CD62L+/CCR7+ phenotype and decreased the CCR7 expression and proportion of DCs in SLOs of aGvHD mice. In conclusion, the altered migratory properties of T cells and DCs might contribute to the immunosuppressive activity of transplanted MSCs in the setting of aGvHD.
Disclosure of potential conflicts of interest is found at the end of this article.