Regulation of Embryonic Stem Cell Self-Renewal and Pluripotency by Foxd3
Version of Record online: 24 JUL 2008
Copyright © 2008 AlphaMed Press
Volume 26, Issue 10, pages 2475–2484, October 2008
How to Cite
Liu, Y. and Labosky, P. A. (2008), Regulation of Embryonic Stem Cell Self-Renewal and Pluripotency by Foxd3. STEM CELLS, 26: 2475–2484. doi: 10.1634/stemcells.2008-0269
- Issue online: 9 JAN 2009
- Version of Record online: 24 JUL 2008
- Manuscript Accepted: 12 JUL 2008
- Manuscript Received: 17 MAR 2008
- Embryonic stem cell;
- Transcription factor
The Foxd3 forkhead transcription factor is required for maintaining pluripotent cells in the early mouse embryo and for the establishment of murine embryonic stem cell (ESC) lines. To begin to understand the role of Foxd3 in ESC maintenance, we derived ESC lines from blastocysts that carried two conditional Foxd3 alleles and a tamoxifen-inducible Cre transgene. Tamoxifen treatment produced a rapid and near complete loss of Foxd3 mRNA and protein. Foxd3-deficient ESCs maintained a normal proliferation rate but displayed increased apoptosis, and clonally dispersed ESCs showed a decreased ability to self-renew. Under either self-renewal or differentiation-promoting culture conditions we observed a strong, precocious differentiation of Foxd3 mutant ESCs along multiple lineages, including trophectoderm, endoderm, and mesendoderm. This profound alteration in biological behavior occurred in the face of continued expression of factors known to induce pluripotency, including Oct4, Sox2, and Nanog. We present a model for the role of Foxd3 in repressing differentiation, promoting self-renewal, and maintaining survival of mouse ESCs.
Disclosure of potential conflicts of interest is found at the end of this article.