IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model§

Authors

  • Kyu-Sup Cho,

    1. Department of Otorhinolaryngology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea
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  • Hye-Kyung Park,

    1. Department of Internal Medicine, Institute of Allergy and Clinical Immunology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea
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  • Hee-Young Park,

    1. Department of Otorhinolaryngology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea
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  • Jin Sup Jung,

    1. Department of Physiology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea
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  • Seong-Gyu Jeon,

    1. Department of Life Science, Postech Biotech Center, Pohang University of Science and Technology, Pohang, Korea
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  • Yoon-Keun Kim,

    1. Department of Life Science, Postech Biotech Center, Pohang University of Science and Technology, Pohang, Korea
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  • Hwan Jung Roh

    Corresponding author
    1. Department of Otorhinolaryngology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea
    • Department of Otorhinolaryngology, Medical Research Institute, Pusan National University School of Medicine, Busan, Korea, 1-10 Ami-dong, Seo-gu, Busan 602-739, Korea
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    • Telephone: 82-51-240-7333; Fax: 82-51-248-1248


  • Author contributions: K.S.C.: collection and/or assembly of data, manuscript writing, data analysis and interpretation; H.K.P.: Collection and/or assembly of data, data analysis and interpretation; H.Y.P.: collection and/or assembly of data; J.S.J.: provision of study material or patients; S.G.J.: data analysis and interpretation; Y.K.K.: provision of study material or patients; H.J.R.: conception and design.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSExpress October 2, 2008.

Abstract

Adipose tissue-derived stem cells (ASCs) exhibit immunosuppressive effects in allogeneic transplantation. However, there is no report that evaluates the in vivo immune-modulating effect of ASCs in an experimental allergic rhinitis (AR) model. We investigated whether ASCs migrate to the nasal mucosa in an AR mouse model and evaluated the immune-modulating effect of ASCs in the AR mouse model. Cultured ASCs (2 × 106) were injected i.v. before the first allergen challenge in the AR mouse model. Migration of ASCs to the nasal mucosa was evaluated by immunofluorescence. The immunomodulatory effects of ASCs were evaluated by nasal symptoms, histology, serum ovalbumin (OVA)-specific antibody, and the cytokine profile of the spleen. ASCs migrated to the nasal mucosa in the AR mouse model. ASCs significantly reduced allergic symptoms and inhibited eosinophilic inflammation in the nasal mucosa. ASCs significantly decreased the serum allergen-specific IgE level and the IgG1/IgG2a ratio and significantly increased the IgG2a level in the AR mouse model. ASCs inhibited interleukin (IL)-4 and IL-5 production from OVA-incubated splenocytes, but enhanced interferon-γ production. In conclusion, ASCs can migrate to the nasal mucosa in the AR mouse model and inhibit eosinophilic inflammation partly via shifting to a T-helper 1 (Th1) from a Th2 immune response to allergens. STEM CELLS2009;27:259–265

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