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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 5 JAN 2009
Copyright © 2008 AlphaMed Press
Volume 27, Issue 1, pages 49–58, January 2009
How to Cite
Suter, D. M., Tirefort, D., Julien, S. and Krause, K.-H. (2009), A Sox1 to Pax6 Switch Drives Neuroectoderm to Radial Glia Progression During Differentiation of Mouse Embryonic Stem Cells. STEM CELLS, 27: 49–58. doi: 10.1634/stemcells.2008-0319
Author contributions: D.M.S.: conception and design, collection and assembly of the data, data analysis and interpretation, manuscript writing; D.T. and S.J.: collection and assembly of the data; K.-H.K.: conception and design, manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSExpress September 25, 2008.
- Issue published online: 5 JAN 2009
- Article first published online: 5 JAN 2009
- Manuscript Accepted: 16 SEP 2008
- Manuscript Received: 29 MAR 2008
- Swiss National Science Foundation (NFP46). Grant Number: 404640-101109
- Louis Jeantet Foundation
- M.D./Ph.D. Scholarship from the Swiss National Science Foundation
- Radial glia;
- Embryonic stem cells
The transcription factors Sox1 and Pax6 are expressed sequentially during early mouse embryonic neurogenesis. Sox1 expression starts upon formation of neuroectoderm, whereas Pax6 is subsequently expressed in radial glial cells, the latter giving rise to most neurons of the cerebral cortex. Here we used mouse embryonic stem (ES) cells to study the role of Sox1 and Pax6 in regulating differentiation of neural progenitors. For this purpose, we investigated the effect of overexpression and knockdown of Sox1 and Pax6, using three differentiation protocols. We show that (a) expression of Sox1 or Pax6 in uncommitted ES cells favored neuroectodermal lineage choice; (b) continuous Sox1 expression maintained cells at the neuroepithelial stage and prevented expression of Pax6 and other radial glial cell markers; (c) Sox1 knockdown facilitated exit from the progenitor stage, whereas Pax6 knockdown decreased formation of radial glia; (d) forced Pax6 expression in neuroepithelial cells triggered their differentiation into radial glia and neurons; and (e) Pax6 expression induced cell migration, a feature typical of radial glia-derived early neurons. We conclude that Sox1 enhances neuroectodermal commitment and maintenance but blocks further differentiation. In contrast, Pax6 is involved in the progression of neuroectoderm toward radial glia. STEM CELLS2009;27:49–58