• Open Access

Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells§

Authors

  • Nina Kossack,

    1. Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California, USA
    2. Center of Reproductive Medicine and Andrology, University of Muenster, Muenster, Germany
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  • Juanito Meneses,

    1. Center for Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA
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  • Shai Shefi,

    1. Department of Urology, University of California, San Francisco, San Francisco, California, USA
    2. Sheba Medical Center, Tel Hashomer, Israel
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  • Ha Nam Nguyen,

    1. Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California, USA
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  • Shawn Chavez,

    1. Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California, USA
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  • Cory Nicholas,

    1. Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California, USA
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  • Joerg Gromoll,

    1. Center of Reproductive Medicine and Andrology, University of Muenster, Muenster, Germany
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  • Paul J. Turek,

    Corresponding author
    1. Department of Urology, University of California, San Francisco, San Francisco, California, USA
    • Paul J. Turek, University of California, San Francisco, San Francisco, California 94043, USA

      Renee A. Reijo-Pera, Stanford University School of Medicine, Palo Alto, California 94304-5542, USA

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    • Telephone: 415-392-3200

  • Renee A. Reijo-Pera

    Corresponding author
    1. Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California, USA
    • Paul J. Turek, University of California, San Francisco, San Francisco, California 94043, USA

      Renee A. Reijo-Pera, Stanford University School of Medicine, Palo Alto, California 94304-5542, USA

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    • Telephone: 650-725-3803; Fax: 650-736-2961


  • Author contributions: N.K.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing; J.M. and S.S.: collection and/or assembly of data, data analysis and interpretation; H.N.N., S.C., and C.N.: collection and/or assembly of data, data analysis and interpretation, manuscript writing; J.G.: manuscript writing; P.J.T.: conception and design, provision of study material or patients, manuscript writing; R.A.R.-P.: conception and design, financial support, administrative support, data analysis and interpretation, manuscript writing, final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLS Express October 16, 2008; available online without subscription through the open access option.

Abstract

Several reports have documented the derivation of pluripotent cells (multipotent germline stem cells) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogonia-derived stem cells express embryonic stem cell markers and differentiate to the three primary germ layers, as well as the germline. Data indicate that derivation may involve reprogramming of endogenous spermatogonia in culture. Here, we report the derivation of human multipotent germline stem cells (hMGSCs) from a testis biopsy. The cells express distinct markers of pluripotency, form embryoid bodies that contain derivatives of all three germ layers, maintain a normal XY karyotype, are hypomethylated at the H19 locus, and express high levels of telomerase. Teratoma assays indicate the presence of human cells 8 weeks post-transplantation but limited teratoma formation. Thus, these data suggest the potential to derive pluripotent cells from human testis biopsies but indicate a need for novel strategies to optimize hMGSC culture conditions and reprogramming. STEM CELLS 2009;27:138–149

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