Unexpected Multipotency of Melanoblasts Isolated from Murine Skin

Authors

  • Tsutomu Motohashi,

    Corresponding author
    1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan
    • Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
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    • Telephone: +81-58-230-6476, Fax: +81-58-230-6478

  • Katsumasa Yamanaka,

    1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan
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  • Kairi Chiba,

    1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan
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  • Hitomi Aoki,

    1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan
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  • Takahiro Kunisada

    1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan
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  • First published online in STEM CELLSExpress January 8, 2009.

  • Author contributions: T.M.: conception and design, financial support, collection and assembly of data, data analysis and interpretation, manuscript writing; K.Y.: collection and assembly of data; K.C.: collection and assembly of data; H.A.: provision of study materials; T.K.: financial support, administrative support, final approval of manuscript.

Abstract

Melanoblasts, precursor of melanocytes, are generated from the neural crest and differentiate into melanocytes during their migration throughout the entire body. The melanoblasts are thought to be progenitor cells that differentiate only into melanocyte. Here, we show that melanoblasts, even after they have already migrated throughout the skin, are multipotent, being able to generate neurons, glial cells, and smooth muscle cells in addition to melanocytes. We isolated Kit-positive and CD45-negative (Kit+/CD45−) cells from both embryonic and neonate skin by flow cytometry and cultured them on stromal cells. The Kit+/CD45− cells formed colonies containing neurons, glial cells, and smooth muscle cells, together with melanocytes. The Kit+/CD45− cells expressed Mitf-M, Sox10, and Trp-2, which are genes known to be expressed in melanoblasts. Even a single Kit+/CD45− cell formed colonies that contained neurons, glial cells, and melanocytes, confirming their multipotential cell fate. The colonies formed from Kit+/CD45− cells retained Kit+/CD45− cells even after 21 days in culture and these retained cells also differentiated into neurons, glial cells, and melanocytes, confirming their self-renewal capability. When the Kit signal was inhibited by the antagonist ACK2, the Kit+/CD45− cells did not form colonies that contained multidifferentiated cells. These results indicate that melanoblasts isolated from skin have multipotency and self-renewal capabilities. STEM CELLS 2009;27:888–897

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