Translational and Clinical Research

You have full text access to this OnlineOpen article

CD133 Progenitor Cells from the Bone Marrow Contribute to Retinal Pigment Epithelium Repair†‡§

  1. Jeffrey R. Harris,
  2. Robert Fisher,
  3. Marda Jorgensen,
  4. Shalesh Kaushal,
  5. Edward W. Scott¶,*

Article first published online: 2 FEB 2009

DOI: 10.1634/stemcells.2008-0836

Issue

STEM CELLS

STEM CELLS

Volume 27, Issue 2, pages 457–466, February 2009

Additional Information

How to Cite

Harris, J. R., Fisher, R., Jorgensen, M., Kaushal, S. and Scott, E. W. (2009), CD133 Progenitor Cells from the Bone Marrow Contribute to Retinal Pigment Epithelium Repair. STEM CELLS, 27: 457–466. doi: 10.1634/stemcells.2008-0836

Author Information

  1. Program in Stem Cell Biology, McKnight Brain Institute, University of Florida, Gainesville, Florida, USA

  1. Telephone: 352-846-1149; Fax: 352-392-5802

*Program in Stem Cell Biology and Regenerative Medicine, University of Florida, Box 100201, 1600 SW Archer Road, Gainesville, Florida 32610, USA

  1. †

    Author contributions: J.R.H.: conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing; R.F.: data analysis and interpretation, manuscript editing; M.J.: collection of data; S.K.: collection of functional data; E.W.S.: conception and design, financial support, data analysis and interpretation, manuscript writing, final approval of manuscript.

  2. ‡

    Disclosure of potential conflicts of interest is found at the end of this article.

  3. §

    First published online in STEM CELLSExpress November 26, 2008.

  4. Telephone: 352-846-1149; Fax: 352-392-5802

Publication History

  1. Issue published online: 2 FEB 2009
  2. Article first published online: 2 FEB 2009
  3. Manuscript Accepted: 5 NOV 2008
  4. Manuscript Received: 25 AUG 2008

Funded by

  • NIH. Grant Numbers: RO1 EY18158, RO1 HL75258

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (1798K)

Abstract

Our goal was to define a clinically significant population of cells by utilizing a single-step selection process to enrich hematopoietic cells capable of regenerating the retinal pigment epithelium (RPE). Utilizing intravitreal injection of bone marrow cells from a mouse with pigment (C57BL6:gfp) into albino recipient mice (C57BL6:Tyr-), we show that hematopoietic progenitor cells (HPCs) enriched for CD133 can regenerate RPE cells and improve retinal function. The chemokine CXCL12 (stromal cell-derived factor 1α) is essential for migration, incorporation, and RPE regeneration by CD133+ HPCs. Once incorporated, CD133+ HPCs become pigmented, adopt an RPE morphology, and express RPE-specific proteins, leading to partial functional recovery by electroretinogram. Human CD133+ HPCs also incorporate in the retina and assume RPE morphology in nonobese diabetic/severe combined immunodeficient mice xenografts. These data show that a clinically accessible CD133+ hematopoietic cell can home to an injured RPE layer, differentiate into cells with significant RPE morphology, and provide therapeutic functional recovery of the visual cycle. STEM CELLS2009;27:457–466

View Full Article with Supporting Information (HTML) Get PDF (1798K)