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Tissue-Specific Stem Cells
Version of Record online: 2 MAR 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 3, pages 612–622, March 2009
How to Cite
Teisanu, R. M., Lagasse, E., Whitesides, J. F. and Stripp, B. R. (2009), Prospective Isolation of Bronchiolar Stem Cells Based Upon Immunophenotypic and Autofluorescence Characteristics. STEM CELLS, 27: 612–622. doi: 10.1634/stemcells.2008-0838
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: R.M.T.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing; E.L. and J.F.W.: collection and/or assembly of data, data analysis and interpretation; B.R.S.: conception and design, financial support, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript.
First published online in STEM CELLSExpress December 4, 2008.
- Issue online: 2 MAR 2009
- Version of Record online: 2 MAR 2009
- Manuscript Accepted: 21 OCT 2008
- Manuscript Received: 25 AUG 2008
- Bronchiolar stem cell;
- Bronchioalveolar stem cell;
Bronchiolar stem cells have been functionally defined in vivo on the basis of their resistance to chemical (naphthalene) injury, their infrequent proliferation relative to other progenitor cell types, and their coexpression of the airway and alveolar secretory cell markers Clara cell secretory protein and pro-surfactant protein C, respectively. Cell surface markers that have previously been used for their prospective isolation included Sca-1 and CD34. Using transgenic animal models associated with stem cell expansion, ablation, and lineage tracing, we demonstrate that CD34pos cells do not belong to the airway epithelial lineage and that cell surface Sca-1 immunoreactivity does not distinguish between bronchiolar stem and facultative transit-amplifying (Clara) cell populations. Furthermore, we show that high autofluorescence (AFhigh) is a distinguishing characteristic of Clara cells allowing for the fractionation of AFlow bronchiolar stem cells. On the basis of these data we show that the defining phenotype of the bronchiolar stem cell is CD45neg CD31neg CD34neg Sca-llow AFlow. This refinement in the definition of bronchiolar stem cells provides a critical tool by which to assess functional and molecular distinctions between bronchiolar stem cells and the more abundant pool of facultative transit-amplifying (Clara) cells. STEM CELLS2009;27:612–622