Telephone: +91-020-25708078; Fax: +91-020-25692259
Cancer Stem Cells
Article first published online: 2 MAR 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 3, pages 498–508, March 2009
How to Cite
Kusumbe, A. P., Mali, A. M. and Bapat, S. A. (2009), CD133-Expressing Stem Cells Associated with Ovarian Metastases Establish an Endothelial Hierarchy and Contribute to Tumor Vasculature. STEM CELLS, 27: 498–508. doi: 10.1634/stemcells.2008-0868
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSExpress January 8, 2009.
Author contributions: A.P.K.: collection and assembly of data, data analysis and interpretation, manuscript writing; A.M.M.: collection and assembly of data; S.A.B.: conception and design of study, administrative support, provision of study materials, assembly of data, data analysis and interpretation, manuscript writing.
- Issue published online: 2 MAR 2009
- Article first published online: 2 MAR 2009
- Manuscript Accepted: 27 DEC 2008
- Manuscript Received: 4 SEP 2008
- Cellular hierarchy;
- Ovarian cancer;
- Tumor vasculature
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited cell type and cellular events orchestrating the entire phenomenon remains obscure. We report that human ovarian cancer is frequently associated with cells expressing the stem cell surface marker CD133. We further show that these CD133-expressing cells are nontumorigenic in nature, and they augment tumor development through their vasculogenic potential. This cell population is attracted by cancer stem cells (CSCs) and retains a direct physical association within the CSC-derived spheroids. Our study further delineates the contribution of these vasculogenic CD133+ stem cells, termed by us as endothelial stem cells (EnSCs) to the developing tumor vasculature during disease progression. In support of their being stem cells, the EnSCs have a capability of establishing an entire endothelial cell hierarchy. We conclude that such EnSCs play a crucial role in ensuring the development of long-term tumor vasculature to complement CSC-driven tumor development and disease progression. STEM CELLS2009;27:498–508