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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 2 MAR 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 3, pages 559–567, March 2009
How to Cite
Choi, K.-D., Yu, J., Smuga-Otto, K., Salvagiotto, G., Rehrauer, W., Vodyanik, M., Thomson, J. and Slukvin, I. (2009), Hematopoietic and Endothelial Differentiation of Human Induced Pluripotent Stem Cells. STEM CELLS, 27: 559–567. doi: 10.1634/stemcells.2008-0922
First published online in STEM CELLSExpress January 8, 2009.
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: K.-D.C., G.S., and M.V.: collection and assembly of data, conception and design, data analysis; W.R.: collection of data; J.T.: conception, generation of iPSC lines; J.Y. and K.S.-O.: generation of iPSC lines; I.S.: conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript.
- Issue published online: 2 MAR 2009
- Article first published online: 2 MAR 2009
- Manuscript Accepted: 9 DEC 2008
- Manuscript Received: 15 SEP 2008
- Induced pluripotent stem cells;
- Embryonic stem cells;
- Hematopoietic cells;
- Endothelial cells
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well as for developing novel approaches for regenerative therapy based on immunologically compatible cells. In this study, we employed an OP9 differentiation system to characterize the hematopoietic and endothelial differentiation potential of seven human iPSC lines obtained from human fetal, neonatal, and adult fibroblasts through reprogramming with POU5F1, SOX2, NANOG, and LIN28 and compared it with the differentiation potential of five human embryonic stem cell lines (hESC, H1, H7, H9, H13, and H14). Similar to hESCs, all iPSCs generated CD34+CD43+ hematopoietic progenitors and CD31+CD43− endothelial cells in coculture with OP9. When cultured in semisolid media in the presence of hematopoietic growth factors, iPSC-derived primitive blood cells formed all types of hematopoietic colonies, including GEMM colony-forming cells. Human induced pluripotent cells (hiPSCs)-derived CD43+ cells could be separated into the following phenotypically defined subsets of primitive hematopoietic cells: CD43+CD235a+CD41a± (erythro-megakaryopoietic), lin−CD34+CD43+CD45− (multipotent), and lin−CD34+CD43+CD45+ (myeloid-skewed) cells. Although we observed some variations in the efficiency of hematopoietic differentiation between different hiPSCs, the pattern of differentiation was very similar in all seven tested lines obtained through reprogramming of human fetal, neonatal, or adult fibroblasts with three or four genes. Although several issues remain to be resolved before iPSC-derived blood cells can be administered to humans for therapeutic purposes, patient-specific iPSCs can already be used for characterization of mechanisms of blood diseases and for identification of molecules that can correct affected genetic networks. STEM CELLS2009;27:559–567