• 1
    Kaushansky K, Lok S, Holly RD, et al. Promotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombopoietin. Nature 1994; 369: 568571.
  • 2
    Wendling F, Maraskovsky E, Debili N, et al. cMpl ligand is a humoral regulator of megakaryocytopoiesis. Nature 1994; 369: 571574.
  • 3
    Banu N, Wang J, Deng B, et al. Modulation of megakaryocytopoiesis by thrombopoietin: the c-Mpl ligand. Blood 1995; 86: 13311338.
  • 4
    Zeigler FC, de Sauvage F, Widmer HR, et al. In vitro megakaryocytopoietic and thrombopoietic activity of c-mpl ligand (TPO) on purified murine hematopoietic stem cells. Blood 1994; 84: 40454052.
  • 5
    Kobayashi M, Laver JH, Kato T, et al. Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3. Blood 1996; 88: 429436.
  • 6
    Ku H, Yonemura Y, Kaushansky K, et al. Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation of primitive hematopoietic progenitors of mice. Blood 1996; 87: 45444551.
  • 7
    Young JC, Bruno E, Luens KM, et al. Thrombopoietin stimulates megakaryocytopoiesis, myelopoiesis, and expansion of CD34+ progenitor cells from single CD34+Thy-1+Lin primitive progenitor cells. Blood 1996; 88: 16191631.
  • 8
    Ramsfjell V, Borge OJ, Cui L, et al. Thrombopoietin directly and potently stimulates multilineage growth and progenitor cell expansion from primitive (CD34+CD38) human bone marrow progenitor cells: distinct and key interactions with the ligands for c-kit and flt3, and inhibitory effects of TGF-beta and TNF-alpha. J Immunol 1997; 158: 51695177.
  • 9
    Yagi M, Ritchie KA, Sitnicka E, et al. Sustained ex vivo expansion of hematopoietic stem cells mediated by thrombopoietin. Proc Natl Acad Sci USA 1999; 96: 81268131.
  • 10
    Emma H, Takano H, Sudo K, et al. In vitro self-renewal division of hematopoietic stem cells. J Exp Med 2000; 192: 12811288.
  • 11
    Carver-Moore K, Broxmeyer HE, Luoh SM, et al. Low levels of erythroid and myeloid progenitors in thrombopoietin- and c-mpl-deficient mice. Blood 1996; 88: 803808.
  • 12
    de Sauvage FJ, Carver-Moore K, Luoh SM, et al. Physiological regulation of early and late stages of megakaryocytopoiesis by thrombopoietin. J Exp Med 1996; 183: 651656.
  • 13
    Gurney AL, Carver-Moore K, de Sauvage FJ, et al. Thrombocytopenia in c-mpl-deficient mice. Science 1994; 265: 14451447.
  • 14
    Alexander WS, Roberts AW, Nicola NA, et al. Deficiencies in progenitor cells of multiple hematopoietic lineages and defective megakaryocytopoiesis in mice lacking the thrombopoietin receptor c-Mpl. Blood 1996; 87: 21622170.
  • 15
    Kimura S, Roberts AW, Metcalf D, et al. Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietin. Proc Natl Acad Sci USA 1998; 95: 11951200.
  • 16
    Methia N, Louache F, Vainchenker W, et al. Oligodeoxynucleotides antisense to the proto-oncogene c-mpl specifically inhibit in vitro megakaryocytopoiesis. Blood 1993; 82: 13951401.
  • 17
    Berardi AC, Wang A, Levine JD, et al. Functional isolation and characterization of human hematopoietic stem cells. Science 1995; 267: 104108.
  • 18
    Solar GP, Kerr WG, Zeigler FC, et al. Role of c-mpl in early hematopoiesis. Blood 1998; 92: 410.
  • 19
    Brizzi MF, Battaglia E, Montrucchio G, et al. Thrombopoietin stimulates endothelial cell motility and neoangiogenesis by a platelet-activating factor-dependent mechanism. Circ Res 1999; 84: 785796.
  • 20
    Cardier JE, Dempsey J. Thrombopoietin and its receptor, c-mpl, are constitutively expressed by mouse liver endothelial cells: evidence of thrombopoietin as a growth factor for liver endothelial cells. Blood 1998; 91: 923929.
  • 21
    Orkin S. GATA-binding transcription factors in hematopoietic cells. Blood 1992; 80: 575581.
  • 22
    Yamaguchi TP, Dumont DJ, Conlon RA, et al. Flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors. Development 1993; 118: 489498.
  • 23
    Kallianpur AR, Jordan JE, Brandt SJ. The SCL/TAL-1 gene is expressed in progenitors of both the hematopoietic and vascular systems during embryogenesis. Blood 1994; 83: 12001208.
  • 24
    Young PE, Baumhueter S, Lasky LA. The sialomucin CD34 is expressed on hematopoietic cells and blood vessels during murine development. Blood 1995; 85: 96105.
  • 25
    Kabrun N, Buhring HJ, Choi K, et al. Flk-1 expression defines a population of early embryonic hematopoietic precrusors. Development 1997; 124: 20392048.
  • 26
    His W. Lecithoblast und angioblast der wirbelthiere. Abhandl. KS Ges Wiss Math Phys 1900; 22: 171328.
  • 27
    Sabin FR. Studies on the origin of blood vessels and of red blood corpuscles as seen in the living blastoderm of chicks during the second day of incubation. Contrib Embryol 1920; 9: 213262.
  • 28
    Kennedy M, Firpo M, Choi K, et al. A common precursor for primitive erythropoiesis and definitive hematopoiesis. Nature 1997; 386: 488493.
  • 29
    Choi K, Kennedy M, Kazarov A, et al. A common precursor for hematopoietic and endothelial cells. Development 1998; 125: 725732.
  • 30
    Robertson EJ. Embryo-derived stem cell lines. In: RJRobertson, ed. Teratocarcinomas and Embryonic Stem Cells: A Practical Approach. Oxford: IRL Press, 1987:71112.
  • 31
    Jim L, Siritanaratkul N, Emery DW, et al. Targeted expansion of genetically modified bone marrow cells. Proc Natl Acad Sci USA 1998; 95: 80938097.
  • 32
    Jin L, Zeng H, Chien S, et al. In vivo selection using a cell-growth switch. Nat Genet 2000; 26: 6466.
  • 33
    Grignani F, Kinsella T, Mencarelli A, et al. High-efficiency gene transfer and selection of human hematopoietic progenitor cells with a hybrid EBV/retroviral vector expressing the green fluorescence protein. Cancer Res 1998; 58: 1419.
  • 34
    Brady G, Barbara M, Iscove N. Representative in vitro cDNA amplification from individual hemopoietic cells and colonies. Methods Mol Cell Biol 1990; 2: 1725.
  • 35
    Kieran MW, Perkins AC, Orkin SH, et al. Thrombopoietin rescues in vitro erythroid colony formation from mouse embryos lacking the erythropoietin receptor. Proc Natl Acad Sci USA 1996; 93: 91269131.
  • 36
    Bobik R, Hong Y, Breier G, et al. Thrombopoietin stimulates VEGF release from c-Mpl-expressing cell lines and haematopoietic progenitors. FEBS Lett 1998; 423: 1014.
  • 37
    Newman PJ. The role of PECAM-1 in vascular cell biology. Ann NY Acad Sci 1994; 714: 165174.
  • 38
    Voyta JC, Via DP, Butterfield CE, et al. Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol 1984; 99: 20342040.
  • 39
    Williams RL, Courtneidge SA, Wagner EF. Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell 1988; 52: 121131.
  • 40
    Suri C, Jones PF, Patan S, et al. Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell 1996; 87: 11711180.
  • 41
    Watt SM, Gschmeissner SE, Bates PA. PECAM-1: its expression and function as a cell adhesion molecule on hematopoietic and endothelial cells. Leuk Lymphoma 1995; 17: 229244.
  • 42
    Nishikawa SI, Nishikawa S, Hirashima M, et al. Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. Development 1998; 125: 17471757.
  • 43
    Shalaby F, Rossant J, Yamaguchi TP, et al. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 1995; 376: 6266.
  • 44
    Carmeliet P, Ferreira V, Breier G, et al. Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. Nature 1996; 380: 435439.
  • 45
    Ferrara N, Carver-Moore K, Chen H, et al. Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene. Nature 1996; 380: 439442.
  • 46
    Schuh AC, Faloon P, Hu QL, et al. In vitro hematopoietic and endothelial potential of flk-1−/− embryonic stem cells and embryos. Proc Natl Acad Sci USA 1999; 96: 21592164.
  • 47
    Filippi MD, Porteu F, Le Pesteur F, et al. Embryonic stem cell differentiation to hematopoietic cells: a model to study the function of various regions of the intracytoplasmic domain of cytokine receptors in vitro. Exp Hematol 2000; 28: 13631372.
  • 48
    Filippi MD, Porteu F, Le Pesteur F, et al. Requirement for mitogen-activated protein kinase activation in the response of embryonic stem cell-derived hematopoietic cells to thrombopoietin in vitro. Blood 2002; 99: 11741182.
  • 49
    Petzer AL, Zandstra PW, Piret JM, et al. Differential cytokine effects on primitive (CD34+CD38) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin. J Exp Med 1996; 183: 25512558.
  • 50
    Zeng H, Masuko M, Jin L, et al. Receptor specificity in the self-renewal and differentiation of primary multipotential hemopoietic cells. Blood 2001; 98: 328334.
  • 51
    Winnier G, Blessing M, Labosky PA, et al. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev 1995; 9: 21052116.
  • 52
    Johansson BM, Wiles MV. Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development. Mol Cell Biol 1995; 15: 141151.
  • 53
    Dickson MC, Martin JS, Cousins FM, et al. Defective haematopoiesis and vasculogenesis in transforming growth factor-beta 1 knock out mice. Development 1995; 121: 18451854.