These authors contributed equally.
Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells
Article first published online: 1 MAY 2004
Copyright © 2004 AlphaMed Press
Volume 22, Issue 3, pages 367–376, May 2004
How to Cite
Heins, N., Englund, M. C.O., Sjöblom, C., Dahl, U., Tonning, A., Bergh, C., Lindahl, A., Hanson, C. and Semb, H. (2004), Derivation, Characterization, and Differentiation of Human Embryonic Stem Cells. STEM CELLS, 22: 367–376. doi: 10.1634/stemcells.22-3-367
- Issue published online: 23 DEC 2008
- Article first published online: 1 MAY 2004
- Manuscript Accepted: 1 DEC 2003
- Manuscript Received: 1 AUG 2003
- Human blastocyst;
- Human ES cell;
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronase-treated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propagated in an undifferentiated pluripotent state.