Breaking the Species Barrier: Derivation of Germline-Competent Embryonic Stem Cells from Mus spretus × C57BL/6 Hybrids

Authors

  • Tino Hochepied,

    1. Molecular Genetics of the Mouse, Department for Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
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  • Luc Schoonjans,

    1. Thromb-X N.V., Center for Molecular and Vascular Biology, Catholic University of Leuven, Leuven, Belgium
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  • Jan Staelens,

    1. Molecular Genetics of the Mouse, Department for Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
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  • Veerle Kreemers,

    1. Thromb-X N.V., Center for Molecular and Vascular Biology, Catholic University of Leuven, Leuven, Belgium
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  • Sophie Danloy,

    1. Thromb-X N.V., Center for Molecular and Vascular Biology, Catholic University of Leuven, Leuven, Belgium
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  • Leen Puimège,

    1. Molecular Genetics of the Mouse, Department for Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
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  • Désiré Collen,

    1. Thromb-X N.V., Center for Molecular and Vascular Biology, Catholic University of Leuven, Leuven, Belgium
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  • Frans Van Roy,

    1. Molecular Genetics of the Mouse, Department for Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
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  • Claude Libert Ph.D.

    Corresponding author
    1. Molecular Genetics of the Mouse, Department for Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
    • Department of Molecular and Biomedical Research, University of Ghent/VIB, Technologiepark 927, B-9052 Zwijnaarde, Belgium. Telephone: 00-32-9-331-3700; Fax: 00-32-9-331-3609
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Abstract

Embryonic stem (ES) cells, which can differentiate into almost all types of cells, have been derived from the house mouse Mus musculus, rat, rabbit, humans, and other species. Transmission of the genotype to the offspring of chimeras has been achieved only with M. musculus ES cells, limiting targeted mutagenesis using ES cells to this species. Mus spretus, which exhibits many genetic polymorphisms with M. musculus, displays dominant resistance to cancer and inflammation, making derived inbred strains very useful in positional cloning and interspecies mapping. We show here for the first time the derivation of ES cells from hybrid blastocysts, obtained by the mating of two different species, namely Mus musculus and Mus spretus, and their use for the generation of chimeric mice that transmit the Mus spretus genotype and phenotype to the offspring. These hybrid ES cells allow the genetic manipulation of Mus spretus, as an alternative to Mus musculus.

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