Antifungal isolates database of amphibian skin-associated bacteria and function against emerging fungal pathogens

Ecological Archives E096-059

Authors

  • Douglas C. Woodhams,

    Corresponding author
    • Corresponding author. Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado 80309-0334, USA, and Smithsonian Tropical Research Institute, Panama. Present address: Department of Biology, University of Massachusetts, Boston, Massachusetts, USA. E-mail: dwoodhams@gmail.com See the full data paper in Ecological Archives for affiliations of other authors.

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  • Ross A. Alford,

  • Rachael E. Antwis,

  • Holly Archer,

  • Matthew H. Becker,

  • Lisa K. Belden,

  • Sara C. Bell,

  • Molly Bletz,

  • Joshua H. Daskin,

  • Leyla R. Davis,

  • Sandra V. Flechas,

  • Antje Lauer,

  • Antonio Gonzalez,

  • Reid N. Harris,

  • Whitney M. Holden,

  • Myra C. Hughey,

  • Roberto Ibáñez,

  • Rob Knight,

  • Jordan Kueneman,

  • Falitiana Rabemananjara,

  • Laura K. Reinert,

  • Louise A. Rollins-Smith,

  • Franklin Roman-Rodriguez,

  • Stephanie D. Shaw,

  • Jenifer B. Walke,

  • Valerie McKenzie


  • Corresponding Editor: W. K. Michener.

  • See the full data paper in Ecological Archives for affiliations of other authors.

Abstract

Microbial symbionts of vertebrate skin have an important function in defense of the host against pathogens. In particular, the emerging chytrid fungus Batrachochytrium dendrobatidis, causes widespread disease in amphibians but can be inhibited via secondary metabolites produced by many different skin-associated bacteria. Similarly, the fungal pathogens of terrestrial salamander eggs Mariannaea elegans and Rhizomucor variabilis are also inhibited by a variety of skin-associated bacteria. Indeed, probiotic therapy against fungal diseases is a recent approach in conservation medicine with growing experimental support. We present a comprehensive Antifungal Isolates Database of amphibian skin-associated bacteria that have been cultured, isolated, and tested for antifungal properties. At the start, this database includes nearly 2000 cultured bacterial isolates from 37 amphibian host species across 18 studies on five continents: Africa, Oceania, Europe, and North and South America. As the research community gathers information on additional isolates, the database will be updated periodically. The resulting database can serve as a conservation tool for amphibians and other organisms, and provides empirical data for comparative and bioinformatic studies. The database consists of a FASTA file containing 16S rRNA gene sequences of the bacterial isolates, and a metadata file containing information on the host species, life-stage, geographic region, and antifungal capacity and taxonomic identity of the isolate.

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