Presented at the 12th Annual Meeting, SETAC Europe, Vienna, Austria, May 12–16, 2002.
Symposium: Metals in the Environment: Convened by Almut Gerhardt
Effects of acid mine drainage on larval Chironomus (Diptera, Chironomidae) measured with the multispecies freshwater Biomonitor®†
Article first published online: 5 NOV 2009
Copyright © 2004 SETAC
Environmental Toxicology and Chemistry
Volume 23, Issue 5, pages 1123–1128, May 2004
How to Cite
De Bisthoven, L. J., Gerhardt, A. and Soares, A. M. V. M. (2004), Effects of acid mine drainage on larval Chironomus (Diptera, Chironomidae) measured with the multispecies freshwater Biomonitor®. Environmental Toxicology and Chemistry, 23: 1123–1128. doi: 10.1897/02-603
- Issue published online: 5 NOV 2009
- Article first published online: 5 NOV 2009
- Manuscript Accepted: 14 OCT 2003
- Manuscript Received: 10 DEC 2002
- Aid mine drainage;
- Online biomonitoring
The abandoned São Domingos mine (Portugal) offers a pH and metal gradient of acid mine drainage (AMD), and is an ideal model for ecotoxicological studies. Short-term 24-h bioassays with water from the AMD (pH 3.3, 4.4, and 5.5, and control) were performed with fourth instars of Chironomus in the laboratory and in situ (AMD at pH 5.5) in artificial flow-through channels. This was compared to reference water acidified to the respective pH values (acid only). Behavioral stress responses were monitored online with the Multispecies Freshwater Biomonitor® (MFB). The exposure to AMD and acid only was in the sublethal range (mortality 0–20%). The use of MFB chambers did not affect survival. Stress behavior of Chironomus consisted mainly of decreased locomotory activity in AMD and increased activity in acid-only tests, indicating that the metals in the AMD played a role as stress factor. Field exposure in the AMD mixing zone (pH 5.5) generated similar activity as in the corresponding laboratory exposure.