• Aquatic invertebrates;
  • Fluoranthene;
  • Pentachlorobenzene;
  • Sublethal body residue


The whole-body residues of pentachlorobenzene (PCBz) and fluoranthene (FLU) in Hyalella azteca and Chironomus tentans were determined for a variety of chronic sublethal effects. The endpoints evaluated for H. azteca included 28-d growth and survival and 42-d growth, survival, and reproduction. Adverse effects to C. tentans also were determined at multiple endpoints including 10-d growth, cumulative pupation and emergence, and reproduction. The lowest-observed-effect residue (LOER) based on whole-body residues associated with growth was consistent between compounds and species tested with concentrations ranging from 0.17 to 0.33 μmol/g. For H. azteca, the most sensitive endpoints were growth at 0.23 μmol/g and reproduction at 0.11 μmol/g for PCBz and FLU, respectively. For C. tentans, the most sensitive endpoints were emergence, development and reproduction at 0.02 μmol/g, and development and reproduction at 0.15 μmol/g for PCBz and FLU, respectively. Compared to residues associated with acute lethality, the most sensitive sublethal endpoints were approximately 4 and 60 times lower for PCBz and FLU, respectively. The relative consistency of the sublethal endpoints suggests that body residues can be a valuable tool to evaluate bioaccumulation data as part of a risk assessment to predict adverse effects to biota.