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Comparison of chemical binding to recombinant fathead minnow and human estrogen receptors alpha in whole cell and cell-free binding assays

Authors

  • Cynthia V. Rider,

    1. Department of Molecular Biomedical Sciences, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606, USA
    2. Endocrinology Branch, Reproductive Toxicology Division, U.S. Environmental Protection Agency, MD-72, Research Triangle Park, North Carolina 27711
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  • Phillip C. Hartig,

    1. Endocrinology Branch, Reproductive Toxicology Division, U.S. Environmental Protection Agency, MD-72, Research Triangle Park, North Carolina 27711
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  • Mary C. Cardon,

    1. Endocrinology Branch, Reproductive Toxicology Division, U.S. Environmental Protection Agency, MD-72, Research Triangle Park, North Carolina 27711
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  • Vickie S. Wilson

    Corresponding author
    1. Endocrinology Branch, Reproductive Toxicology Division, U.S. Environmental Protection Agency, MD-72, Research Triangle Park, North Carolina 27711
    • Endocrinology Branch, Reproductive Toxicology Division, U.S. Environmental Protection Agency, MD-72, Research Triangle Park, North Carolina 27711
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  • Published on the Web 5/19/2009.

Abstract

Little evidence shows in vivo effects of phthalates on reproductive endpoints in fish. While most of the evidence is negative [24,25], a few studies report in vivo effects of phthalates on estrogen-dependent endpoints. Christiansen et al. found that BBP, but not DBP, induced vitellogenin in rainbow trout at concentrations of 500 and 1,000 mg/kg administered via intraperitoneal injection [26]. Kim et al. found that DEHP elicited potentially antiestrogenic effects in female medaka, as evidenced by decreased vitellogenin levels, decreased gonadal-somatic indices, and immature oocytes in DEHP-treated females as compared to their control counter-parts [27].

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