Soybean BPS-CV127-9 contains a single insertion locus of the csr1-2 gene. Stability of the genetic modification was demonstrated. The expression of the acetohydroxyacid synthase large sub-unit from Arabidopsis thaliana, conferring tolerance to imidazolinone herbicides, was sufficiently analysed. Bioinformatic analyses did not raise safety issues. No differences were identified in the seed composition that would require further assessment with regard to safety. Regarding agronomic and phenotypic characteristics, a difference in seed weight was identified; however, this difference does not affect the overall safety of this soybean. Although the EFSA GMO Panel cannot conclude on its forage composition, soybean forage is not expected to be imported in significant amount for feed uses. Safety assessment identified no concerns regarding the potential toxicity and allergenicity of the newly expressed proteins or soybean BPS-CV127-9. Compositional data indicating that soybean BPS-CV127-9 is as nutritious as non-GM soybean varieties were supported by the outcome of a chicken feeding study. There are no indications of an increased likelihood of spread and establishment of feral soybean BPS-CV127-9 plants, unless they are exposed to imidazolinone-containing herbicides. Risks associated with an unlikely, but theoretically possible, horizontal transfer of recombinant genes from soybean BPS-CV127-9 to bacteria have not been identified. Considering the scope of this application, interactions with the biotic and abiotic environment are not considered to be a relevant issue. The post-market environmental monitoring plan and reporting intervals are in line with the scope of this application. In conclusion, the EFSA GMO Panel considers that the information available for soybean BPS-CV127-9 addresses scientific comments raised by Member States and that the soybean BPS-CV127-9, as described in this application, is as safe and nutritious as its conventional counterpart and commercial soybean varieties with respect to potential effects on human and animal health and the environment in the context of its intended uses.