SEARCH

SEARCH BY CITATION

Abstract

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References

ABSTRACT Immunotherapy of cancer has been explored for over a century, but it is only in the last decade that various antibody-based products have been introduced into the management of patients with diverse cancers. At present, this is one of the most active areas of clinical research, with eight therapeutic products already approved in oncology. Antibodies against tumor-associated markers have been a part of medical practice in immunohistology and in vitro immunoassays for several decades, have even been used as radioconjugates in diagnostic imaging, and are now becoming increasingly recognized as important biological agents for the detection and treatment of cancer. Molecular engineering has improved the prospects for such antibody-based therapeutics, resulting in different constructs and humanized/human antibodies that can be administered frequently. Consequently, a renewed interest in the development of antibodies conjugated with radionuclides, drugs, and toxins has emerged. We review how antibodies and immunoconjugates have influenced cancer detection and therapy, and also describe promising new developments and challenges for broader applications.


INTRODUCTION

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References

The search for a mechanism to target diseases selectively was first realized when resistance to infectious disease could be transferred from one animal to another through their serum, a process known as passive serotherapy.1 Five years later, in 1895, Hericourt and Richet immunized dogs with a human sarcoma and then transferred the serum to patients.2 This anticipated the “magic bullet” concept of Paul Ehrlich in 1908, that “toxins” could be targeted to cancer and other diseases.3 Another half-century passed before antibodies were identified as the substance in serum responsible for these effects.

Despite being potent immune system instigators for killing infectious agents, clinical research initially focused on immunoconjugates prepared with radionuclides, drugs, or toxins, since unconjugated or “naked” antibodies had little therapeutic benefit in oncology compared with the immunoconjugates. Early immunotherapy trials failed to show substantial responses,4–6, [5], [6] but antibodies against carcinoembryonic antigen (CEA) could selectively target and disclose sites of CEA-expressing cancers in patients, and also deliver cytotoxic radioactivity in human colonic cancer xenografts having CEA.7,8, [8] Thereafter, DeNardo, et al.9 reported responses in lymphoma patients to radiolabeled antibodies, and soon others confirmed that radiolabeled antibodies had antitumor activity in non-Hodgkin lymphoma (NHL), but there was also early evidence that the naked antibodies themselves might be effective.10–12, [11], [12] It was during this same period that rituximab (Rituxan, Genentech, and biogen idec), an anti-CD20 IgG, became of interest as a therapeutic for NHL without being radiolabeled.13 The experience and subsequent introduction of rituximab into the treatment of NHL can be credited for the expanded interest in unconjugated antibodies for cancer therapy.

Antibodies (eg, IgG, which is the most commonly used immunoglobulin form, Figure 1) are unique proteins with dual functionality. All naturally occurring antibodies are multivalent, with IgG having two binding ‘arms.’ Antigen-binding specificity is encoded by three complementarity-determining regions (CDRs), while the Fc-region is responsible for binding to serum proteins (eg, complement) or cells. An antibody itself usually is not responsible for killing target cells, but instead marks the cells that other components or effector cells of the body's immune system should attack, or it can initiate signaling mechanisms in the targeted cell that leads to the cell's self-destruction (Figure 2). The former two attack mechanisms are referred to as antibody-dependent complement-mediated cytotoxicity (CMC) and antibody-dependent cellular cytotoxicity (ADCC). ADCC involves the recognition of the antibody by immune cells that engage the antibody-marked cells and either through their direct action, or through the recruitment of other cell types, lead to the tagged-cell's death. CMC is a process where a cascade of different complement proteins become activated, usually when several IgGs are in close proximity to each other, either with one direct outcome being cell lysis, or one indirect outcome being attracting other immune cells to this location for effector cell function.

thumbnail image

Figure FIGURE 1. Schematic representation of an IgG molecule, its chemically produced fragments, and several recombinant antibody fragments with their nominal molecular weights. At the bottom, a schematic representation of the process involved in engineering murine MAbs to reduce their immunogenicity is provided. A chimeric antibody splices the VL and VH portions of the murine IgG to a human IgG. A humanized antibody splices only the CDR portions from the murine MAb, along with some of the adjacent “framework” regions to help maintain the conformational structure of the CDRs. A fully human IgG can be isolated from specialized transgenic mice bred to produce human IgG after immunizing with tumor antigen or by a specialized phage display method.

Download figure to PowerPoint

thumbnail image

Figure FIGURE 2. Mechanisms of action associated with unconjugated antibodies. In this example, the antigen is shown to be floating in lipid rafts within the tumor cell membrane. (A) Antibodies can activate apoptotic signals by cross-linking antigen, particularly across different lipid rafts. Additional cross-linking of antibody by immune cells can also enhance cellular signaling. (B) Immune cells themselves can attack the antibody-coated cell (eg, phagocytosis), and/or they can liberate additional factors, such as cytokines that attract other cytotoxic cells. (C) If antibodies are positioned closely together, they can initiate the complement cascade that can disrupt the membrane, but some of the complement components also are chemo-attractants for immune effector cells and stimulate blood flow. (D) Tumors also can produce angiogenic factors that initiate neovascularization. Antibodies can neutralize these substances by binding to them, or they can bind directly to unique antigens presented in the new blood vessels, where they could exert similar activities.

Download figure to PowerPoint

Antibodies, when bound to key substances found on the cell surface, also can induce cells to undergo programmed cell death, or apoptosis (Figure 2). For example, if rituximab binds to two CD20 molecules, this triggers signals inside the cell that can induce apoptosis.14 If rituximab is cross-linked by other antiantibodies, the apoptotic signal is intensified.15 This cross-linking could also occur when the antibody is bound by another immune cell through its Fc-gamma receptors (FcγR). Other antibodies, such as trastuzumab (anti-HER2/neu; Herceptin, Genentech) and cetuximab (antiepidermal growth factor receptor, EGFR; Erbitux, ImClone Systems and Bristol-Myers Squibb) also have the ability to inhibit cell proliferation.16–18, [17], [18] Because cells frequently have alternative pathways for critical functions, interrupting a single signaling pathway alone might not be sufficient to ensure cell death. From this perspective, it is not surprising that antibodies are often best used in combination with chemotherapy and radiation therapy to augment their antitumor effects.19–21, [20], [21]

Bevacizumab (Avastin, Genentech) is yet another example of how antibodies can be used therapeutically. This antibody binds to vascular endothelial growth factor (VEGF) that is made by tumor cells to promote vessel formation, thereby preventing it from interacting with endothelial cells to form new blood vessels (Figure 2).22 Antibodies can also be used to modulate immune response. Antibodies to the cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) stimulate T-cell immune responses by blocking the inhibitory effects of CTLA-4, which can enhance tumor rejection.23 However, release of this innate inhibitory mechanism can also increase the risk of autoimmunity.24 Two human anti-CTLA-4 antibodies are currently in early clinical trials (MDX-010, Medarex, and CP-675,206, Pfizer), with evidence that they may have activity in melanoma.24 There are already a number of antibodies used or being studied as therapeutic agents in cancer as well as autoimmune diseases (eg, alemtuzumab, daclizumab, infliximab, rituximab, epratuzumab).25–31, [26], [27], [28], [29], [30], [31] Antibodies also can block molecules associated with cell adhesion, thereby inhibiting tumor metastasis.32,33, [33] With such diverse mechanisms of action, there are a number of opportunities for building antibody-based therapeutics.

Antibodies naturally have long serum half-lives. For immunotherapy, this property is helpful because the antibody is maintained in the body fluids, where it can continually interact with its target. For other targeting strategies, most notably with radioconjugates, it can be harmful because the highly radiosensitive bone marrow is continually exposed to radiation, resulting in dose-limiting myelosuppression. The large size of an antibody impacts its ability to move through a tumor mass. A high interstitial pressure inhibits the diffusion of larger molecules within the tumor.34 Migration within the tumor is also inhibited by a binding-site barrier, a process where the antibody as it is leaving the tumor's blood vessels binds to the first available antigen, concentrating the antibody in the perivascular space.35 High-affinity antibodies are less likely to migrate into the tumor bed.36 Administering higher doses of the antibody can reduce the effect of the binding site barrier and allow the antibody to diffuse more deeply into the tumor bed.37 For cytotoxic agents that must be internalized to kill the cell (eg, toxins, cytotoxic drugs), the ability to distribute throughout the tumor is important. Radioconjugates are less affected by this because some radioactive particles can traverse as much as 1.0 cm from where they are deposited (bystander or crossfire effect).

THERAPY WITH UNCONJUGATED ANTIBODIES

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References

A renewed interest in the effects of unconjugated antibodies in cancer began in the early 1980s, after murine monoclonal antibodies (MAbs) became available.38 These initial trials were performed in hematological malignancies, as well as in colorectal cancer and melanoma.4–6,39–41, [5], [6], [39], [40], [41] As with many innovative treatment approaches that are sometimes introduced before the technology has matured sufficiently to extract maximum benefit, only occasional clinical responses were observed. With insufficient efficacy and the immunogenicity of the foreign murine MAb, most of these studies were terminated. Fortunately, some investigators persevered. An excellent lesson on the tribulations of the development of an antibody product between an academic group and industry is that of alemtuzumab (Campath, Berlex, and Genzyme).42 Alemtuzumab (anti-CD52) had one of the earliest and protracted developments of an antibody ultimately commercialized. It took over 20 years from the development of the first rat immunoglobulin against CD52, changing the immunoglobulin type, and finally developing a humanized, recombinant form, and involved several commercial firms during this time. Chemotherapy-refractive chronic lymphocytic leukemia was the indication finally approved in 2001.

Due in part to the contributions made by the groups led by Morrison (Columbia and Stanford Universities) and Winter (Cambridge), MAbs now are engineered to remove a significant portion of the murine component of the IgG, substituting human IgG components before entering clinical studies.43–45, [44], [45] Chimeric antibodies essentially splice VL and VH regions on the murine antibody to the human IgG, making a molecule that is 75% human and 25% murine IgG, whereas a humanized antibody grafts the CDR regions from a murine MAb, along with some of the surrounding “framework” regions to maintain CDR conformation, onto a human IgG, essentially making a molecule with 5% of its sequence from the parental MAb (Figure 1).45 More recent advances have made available, either by genetic or phage-display methods, the development of fully human MAbs that have now entered clinical trials.46 Such engineered MAbs are postulated to greatly reduce the immunogenicity of antibodies, allowing multiple injections to be given, and the human Fc enhances the interaction with other immune system elements.

Rituximab is perhaps the most prominent example of a highly successful paradigm of antibody therapy. As a chimeric antibody, not only did it have reduced immunogenicity, but its effector function (associated with the Fc-portion) was improved. For example, when testing ADCC activity against follicular lymphoma isolated from 43 patients, Weng, et al. reported that only rituximab, not its parent murine anti-CD20 IgG (2B8), had activity in vitro.47 Rituximab was initially approved as a single agent therapy for relapsed or refractory low-grade, follicular B-cell NHL, having an overall response rate of 48% (10% were complete responses, CR) with a median duration of 11.8 months.48,49, [49] Since CD20 is not expressed on precursors B-cells, rituximab induces a depletion of only mature B-cells. Rituximab's major side effects, which are thought to be associated with the activation of complement pathways, occur during or shortly after its infusion. Other less common side effects include symptoms associated with tumor lysis syndrome, severe mucotaneous reactions, renal toxicity, cardiac arrhythmias, hypersensitivity reactions, and reactivation of hepatitis B (primarily when used in combination with chemotherapy).49

Rituximab's activity is unique among cancer treatments because 40% of the patients retreated with rituximab could again respond with a similar duration.50 Extending the duration of rituximab therapy can improve the response rate, particularly the number of complete responses, and its duration. However, whether given as a maintenance regimen or retreating at the first sign of progression, the time to chemotherapy was the same.51 With both approaches having equal benefit, retreatment is generally favored because of the higher expense of a maintenance regimen. Despite the success of rituximab as a monotherapy, there are still a number of patients who do not respond to the initial treatment, and over time, many of those who do will relapse. In an attempt to improve outcome, rituximab has been combined with chemotherapy regimens, including CHOP, CVP, and MCP, as front-line treatments, with very promising results in not only follicular B-cell lymphomas, but also in diffuse large B-cell lymphomas.52,53, [53] Indeed, trials examining front-line combinations of rituximab and chemotherapy have already demonstrated improvements in response rates, time to progression, and event-free survival, and while the overall response rates are promising based on current 2- to 3-year follow-up data, more time will be required to fully appreciate its impact.52 Even in chronic lymphocytic leukemia (CLL), where initial testing of rituximab was disappointing, dose intensification and combinations with chemotherapy have provided significant improvements in response.54,55, [55] Early clinical studies combining rituximab with a humanized anti-CD22, epratuzumab (Immunomedics, Inc.) suggested the potential for additional benefit, particularly in patients with diffuse large B-cell lymphomas.56,57, [57] Studies have also assessed the possible role of an anti-CD80 MAb (galiximab, biogen idec) as a monotherapy in NHL,58,59, [59] and clinical trials are in progress testing its combination with rituximab.

Considerable attention has been devoted to understanding the mechanism of action of rituximab, particularly why some B-cell lymphomas are affected and why not all patients with follicular lymphomas respond. As mentioned earlier, rituximab has been shown to have CMC, ADCC, and apoptotic activity, with the former two mechanisms believed to have the greatest impact, although there are conflicting views of which of these two pathways contributes the most to the response.14,60–66, [60], [61], [62], [63], [64], [65], [66] Studies in transgenic and other mouse models have supported the importance of the Fc-receptor-mediated mechanism of action for rituximab.67,68, [68] These efforts have contributed in part to a better understanding of the role of various Fc receptors found on a variety of immune effector cells (eg, B-cells, neutrophils, natural killer cells, and monocytes) on (in the case of rituximab) the clearance of B-cells, as well as the plasma half-life of antibodies.69 Not only do the various Fc-receptors influence binding, but the absence of certain carbohydrates on the Fc portion of the IgG can affect both ADCC and CMC activities.70,71, [71] Cartron, et al. found that the expression of the homozygous Fc-gamma RIIIa receptor (CD16) 158V genotype correlated with a higher response rate to rituximab, but it did not have an impact on the progression-free survival.72 Weng, et al. found a similar correlation and also noted that the homozygous expression of the Fc-gamma RIIa histidine/histidine genotype correlated independently with a higher response rate, particularly when assessing the response status ≥6 months from treatment.47 By unraveling the molecular basis for antibody cytotoxicity, not only can more effective antibodies be designed, but it could lead to a more rational approach for combinations to enhance activity, such as the finding that G-CSF up-regulates CD64 (Fc-gamma receptor I), which can enhance the binding of neutrophils and monocytes to B-cells coated with rituximab.73 IL-12 has a similar stimulatory effect in mouse models and more recently has been applied clinically with promising results.74,75, [75] These discoveries are also having an impact on the development of antibodies for treating other cancers.76–80, [77], [78], [79], [80]

The approved antibodies listed in Table 1 indicate that immunotherapy is not restricted to hematological malignancies, but includes diverse target antigens and receptors having different biological functions. Trastuzumab is an anti-HER2/neu antibody that has had a major impact on the therapy of breast cancer and is used alone and in combination with drugs.81–83, [82], [83] HER2/neu is overexpressed on a proportion of breast and other cancers, and trastuzumab binds with an extracellular epitope of this target molecule. About 15% of women whose tumors overexpress HER2/neu respond to trastuzumab, but its efficacy is clearly best when used in combination with chemotherapy, where a 25% increase in the median survival (to 29 months) has been reported.81 Further, the addition of this antibody to adjuvant chemotherapy for breast cancer has improved survival markedly.83 Since only a portion of breast cancer patients overexpress HER2/neu and respond to trastuzumab, selection of suitable patients is important. New data are emerging that suggest trastuzumab treatment after adjuvant chemotherapy can have a significant benefit compared with observation, particularly in reducing the rate of distant recurrence.82

Table TABLE 1. FDA-approved Antibodies for the Parenteral Use in Detection and Treatment of Cancer
Generic NameTrade nameAgent/TargetCancer IndicationApproval
  1. *No longer commercially available.

  2. †CLL = chronic lymphocytic leukemia.

  3. ‡SCLC = small cell lung cancer.

  4. §AML = acute myelogenous leukemia.

Unconjugated    
    RituximabRituxanChimeric anti-CD20 IgG1B-cell lymphoma1997
    TrastuzumabHerceptinHumanized anti-HER2 IgG1Breast1998
    AlemtuzumabCampath-1HHumanized anti-CD52CLL2001
    CetuximabErbituxChimeric anti-EGFRColorectalHead/neck20042006
    BevacizumabAvastinChimeric anti-VEGFColorectal2004
Radioconjugates    
    Satumomab pendetideOncoScint*111 In-murine anti-TAG-72 IgGColorectal, ovarian1992
    Nofetumomab merpentanVerluma*99m Tc-murine anti-EGP-1 Fab'SCLC1996
    ArcitumomabCEA-Scan*99m Tc-murine anti-CEA Fab'Colorectal1996
    Capromab pendetideProstaScint111 In-murine anti-PSMAProstate1996
    Ibritumomab tiuxetanZevalin90 Y-murine anti-CD20 IgG + rituximabB-cell lymphoma2002
    TositumomabBexxar131 I-murine anti-CD20 IgG + unlabeled tositumomabB-cell lymphoma2003
Drug conjugates    
    Gemtuzumab ozogamicinMylotargHumanized anti-CD33 IgG4 conjugated to colicheamicinAML§2000

As a member of a family of receptor tyrosine kinases, the binding of HER2 by trastuzumab can interrupt intracellular signaling and affect tumor cell growth. Izumi, et al. showed that trastuzumab also has antiangiogenic properties.84 While this may be an important underlying mechanism of action, other evidence suggests that trastuzumab's activity is principally governed by ADCC.85 However, trastuzumab combined with chemotherapy improves response rates, despite the immunosuppressive activity of the chemotherapy, and trastuzumab's activity is enhanced when combined with other, nonantibody, Erb inhibitors, such as gefitinib and erlotinib, all of which suggest that its ability to interfere with signaling is important.86 Since HER2 is a member of a family of growth factors known as the neuregulins/heregulin and is expressed in multiple neuronal and non-neuronal tissues in embryos and adult animals, including the heart, it is not surprising that cardiomyopathy has been associated with trastuzumab, particularly when combined with paclitaxel and anthracyclines.87–90, [88], [89], [90]

EGFR is also overexpressed in many solid cancers, and when bound by its ligand, cell growth is stimulated. However, when engaged by an EGFR-specific antibody, receptor phosphorylation is decreased and cell growth is inhibited. The chimeric antibody against EGFR, cetuximab, also has an effect on neovascularization.91,92, [92] Cetuximab works best in combination with chemotherapy in colorectal cancer, for which it was initially approved, and with external irradiation in head and neck cancers, which was recently FDA-approved.17,93, [93] Beside the usual risks associated with antibody infusions, cetuximab causes an acneform rash and other skin reactions in most patients, with 10% of these being severe. There is evidence suggesting that the intensity of the skin rash is associated with its antitumor response and even survival.94 Other EGFR antibodies, particularly humanized and fully human forms, also are in development, as indicated in Table 2, and may in fact be better tolerated and show evidence of activity without being combined with cytotoxic chemotherapy, which is currently being evaluated in Phase III trials. It is too early to speculate whether they will, in fact, provide any therapeutic advantages over cetuximab.

Table TABLE 2. Selected Unconjugated Antibody Therapeutics in Advanced Clinical Testing
Generic NameAgent/TargetCancer
  1. *CLL = chronic lymphocytic leukemia.

  2. †CRC = colorectal cancer.

  3. ‡CTCL = cutaneous T-cell lymphoma.

  4. §NHL = non-Hodgkin lymphoma.

  5. ¶NSCLC = non-small cell lung cancer.

  6. **SLL = small lymphocytic lymphomas.

ApolizumabHuman anti-HLA-DRCLL*, SLL**
Chimeric 14.18Chimeric anti-ganglioside (GD2)Neuroblastoma
EpratuzumabHumanized anti-CD22NHL§
GaliximabHumanized anti-CD80NHL§
HuMax-CD4Fully human anti-CD4CTCL
LumiliximabHumanized anti-CD23 (Fc-epsilon RII)CLL*
MDX-010Anti-CTLA-4Melanoma
MatuzumabHumanized anti-EGFRCRC
OrgegovomabMurine anti-CA-125Ovarian
PanitumumabHuman anti-EGFRNSCLC, CRC, renal
PertuzumabHumanized anti-HER-2Breast, prostate, ovarian
RencarexChimeric anti-G250Kidney
VitaxinHumanized anti-αvβ3 integrinMelanoma, prostate

Bevacizumab targets and blocks vascular endothelial growth factor (VEGF) and VEGF's binding to its receptor on the vascular endothelium. Since VEGF is released by many cancers to stimulate proliferation of new blood vessels, the combination of bevacizumab and chemotherapy was found to increase objective responses, median time to progression, and survival in patients with metastatic colorectal cancer, compared with chemotherapy alone, but earlier preclinical studies indicated that anti-VEGF antibodies were active alone, as well as in combination with radiation.22,95,96, [95], [96] It is currently being studied clinically in renal cell, breast, and lung cancers, as well as in a number of other nonhematological and hematological malignancies.97–99, [98], [99] As might be expected, bevacizumab may cause gastrointestinal perforations and delayed wound healing, as well as hemorrhagic events (primarily seen in small cell lung cancer trials, where bevacizumab is not approved). Arterial thromboembolic events (eg, cerebral infarction, transient ischemic attacks, myocardial infarction, angina) and proteinurea also have been reported.100

IMMUNOCONJUGATES

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References

Antibodies also function as carriers of cytotoxic substances, such as radioisotopes, drugs, and toxins (Figure 3). In NHL, anti-CD20 radioconjugates have superior antitumor activity compared with their unconjugated antibody counterparts, but there is increased, albeit manageable, hematological toxicity.101,102, [102] These findings are strong incentives to continue the pursuit of immunoconjugates for cancer therapy.

thumbnail image

Figure FIGURE 3. Immunoconjugates are formed primarily by chemical reactions. Radioconjugates can be formed by coupling radioiodine to tyrosine residues, or by binding chelates to lysine residues, which are then used to bind a variety of radiometals, such as 90 Y. Cysteine residues are also useful for coupling radionuclides, particularly 99m Tc and rhenium, but cysteine is also used for conjugation of drugs and toxins, which can also be coupled to lysine residues. In addition, the carbohydrates found on IgG can be modified to allow the coupling of chelates or drugs. Drugs have also been coupled to an intermediate carrier that allows for a higher number of drugs to be bound to the antibody. Toxin conjugates usually need to be modified to remove their innate cell binding properties, with the biologically active portion then coupled to the antibody or used as a portion of a recombinant fusion protein.

Download figure to PowerPoint

Radionuclides

Radiolabeled antibodies were the first group of immunoconjugates to be examined.103Table 3 lists some of the more commonly used radionuclides conjugated to antibodies for cancer treatment. Because the radioactivity can be detected easily by external scintigraphy, it is also noteworthy to mention the additional application of radiolabeled antibodies for imaging. The demonstration of cancer targeting with a radiolabeled antibody fragment to CEA resulted in the development of radiolabeled antibodies for cancer imaging.7 Since then, 99m Tc- and 111 In-radioconjugates have been commonly used for this application, but with the advent of positron-emission tomography (PET), investigators are now beginning to take advantage of this technologically superior imaging system by radiolabeling tumor-associated antibodies with positron-emitters.104–107, [105], [106], [107]

Table TABLE 3. Physical Properties of Several Examples of Radionuclides Used for Radioconjugate Therapy
Radionuclide
  1. *Assuming a tumor cell is 10 μm in diameter.

131 Iodine
90 Yttrium
177 Lutetium
188 Rhenium
67 Copper
211 Astatine
213 Bismuth
125 Iodine
111 Indium

Whereas external beam radiation delivers a focused beam of high dose rate radiation for short bursts that are divided over several weeks and is designed to treat local disease, radioimmunotherapy (RAIT) is typically given as an intravenous injection, thereby allowing radioactivity to be delivered to tumors throughout the body. Tumor uptake of a radiolabeled IgG occurs gradually, taking 1 to 2 days before peak uptake occurs. Peak uptake is typically <0.01% of the total injected dose per gram tumor, but the radioactivity deposited in the tumor can be detected several weeks later.108 Because of its kinetics, the radiation-absorbed dose delivered by RAIT occurs at a much lower dose rate than external beam irradiation, but is continually present for a period of time defined by the physical half-life of the radionuclide and the biological half-life of the antibody residing in the tumor. This continuous, low dose rate radiation exposure can be as effective as intermittent, high dose rate radiation.109,110, [110]

When it comes to choices of radionuclides for therapy, tumor size is the primary consideration. Medium-energy beta-emitters, such as 131 I (0.5 MeV) and 177 Lu (0.8 MeV), can traverse 1.0 mm, while high-energy beta-emitters, such as 90 Y or 188 Re (2.1 MeV), can penetrate up to 11 mm, making it possible for beta-emitters to kill across several hundred cells, referred to earlier as a bystander or crossfire effect.111 This is considered a significant attribute for radioconjugates compared with other immunoconjugates, since they can be therapeutically active even if heterogeneous antigen expression, tumor architecture, or other factors impede targeting of every cell. Although higher energy beta-emitters have the potential of killing cells across a longer path-length, the absorbed fraction is higher for the lower energy beta-emitters (ie, probability of hitting the nuclear DNA), making them efficient killers. Alpha-emitters, such as 213 Bi and 211 At, traverse only a few cell diameters, but an alpha particle is also a far more efficient (energetic) killer than even a low-energy beta particle, requiring fewer “hits” to damage cellular processes.111 Low-energy electrons, such as are produced by Auger emitters (125 I, 67 Ga, or 111 In, for example) have to be in close contact, preferably inside a cell or in the nucleus, to exert a cytotoxic effect. As one might expect, beta-emitters are most likely best applied in situations where the tumors are ≥0.5 cm in diameter; otherwise a substantial portion of the energy from the radioactive decay will be absorbed in the surrounding normal tissue. The alpha and low-energy electron emitters are best applied when the disease burden is smaller, more localized, or where there may be single or small clusters of cells (eg, leukemia, malignant ascites).112,113, [113]

The primary concern for using radionuclide-labeled IgG is that it remains in the blood for an extended period of time, which continually exposes the highly sensitive red marrow to radiation, resulting in dose-limiting myelosuppression. Smaller forms of the antibodies, such as a F(ab′)2 or Fab', and more recently, molecularly engineered antibody subfragments (Figure 1) with more favorable pharmacokinetic properties, are removed more rapidly from the blood, thereby improving tumor/blood ratios.114,115, [115] There have been reports of improved therapeutic responses using smaller-sized antibodies, but these smaller entities frequently are cleared from the blood by renal filtration, and as a result, many radionuclides (eg, radiometals) become trapped in a higher concentration in the kidneys than in the tumor.116 As a consequence of their more rapid blood clearance, the fraction of the injected activity delivered to the tumor is lower with an antibody fragment than with an IgG.

Multistep pretargeting methods, such as those using bispecific antibodies, represent a promising method for imaging and therapy (Figure 4).117 In this strategy, the bispecific antibody has one arm that binds to the tumor antigen while the second binds to a hapten that is typically incorporated in a small peptide that can be radiolabeled. The unlabeled bispecific antibody is first given time to circulate and bind to the tumor, and once it has cleared from the blood, the radiolabeled peptide is given. The small sized radiolabeled peptide escapes from the vasculature very rapidly, where it can bind to the other arm of the bispecific antibody on the tumor. Within minutes, the rest of the peptide clears from the blood, leaving behind only the peptide that localizes to the bispecific antibody bound to the tumor. This method has been shown in preclinical testing to improve tumor/blood ratios by as much as 40-fold, with tumor uptake increased by as much as 10-fold compared with a directly-radiolabeled antibody fragment.118 This same method can increase the total radiation dose to tumors by 1.5-fold and increase the dose rate by 3-fold, resulting in improved antitumor responses.119 Advances in molecular engineering have greatly enhanced the ability to provide uniform and highly novel pretargeting agents.120,121, [121] Other pretargeting approaches have been studied, each showing improved tumor/blood ratios, as well as improving therapy when compared with directly-radiolabeled antibodies.117 Dosimetry data from a pilot clinical study with 90 Y-biotin pretargeted by a new recombinant streptavidin-anti-TAG-72 antibody are promising, and in other indications, such as medullary thyroid cancer and glioma, encouraging therapeutic results using pretargeting methods have been reported.122–124, [123], [124]

thumbnail image

Figure FIGURE 4. Example of a pretargeting approach using a bsMAb that has one arm capable of binding to a tumor antigen and the other arm specific for a hapten. The bsMAb is allowed to localize the tumor and clear from the blood before a radiolabeled hapten-peptide is given. The radiolabeled hapten-peptide clears from the blood very quickly, leaving behind only the material bound to the tumor. Two hapten-binding sites on the peptide help stabilize the radiolabeled hapten-peptide to the tumor.

Download figure to PowerPoint

Two anti-CD20 IgG-radioconjugates are currently FDA-approved for the treatment of indolent and transformed forms of NHL, 90 Y-ibritumomab tiuxetan (Zevalin, biogen idec) and 131 I-tositumomab (Glaxo SmithKline).125 Both of these treatments improve the objective response rate compared with the unlabeled anti-CD20 antibody used to deliver the radionuclide.101,102, [102] Initially, there was some concern that while objective response rates were significantly improved, the pivotal trial performed with 90 Y-ibritumomab tiuxetan did not show a statistical improvement in the duration of the response compared with its unlabeled antibody (ie, rituximab). However, continued follow up has shown the complete responses have been more durable.126,127, [127] Durable responses have also been reported with 131 I-tositumomab, and importantly, there is evidence that when used as a front-line therapy, it is better tolerated and may improve responses compared with standard chemotherapy.128,129, [129] Clinical studies are also beginning to evaluate the use of 90 Y- ibritumomab tiuxetan as a front-line treatment and are showing these treatments do not preclude patients from receiving additional cytotoxic therapies.130–132, [131], [132] Although more randomized clinical trials (RCT) and long-term follow up to assess the risk for late toxicities (eg, myelodysplasia) are needed, it is impressive that a single treatment with a radiolabeled antibody with fewer side effects than the chemotherapy that is given over several months can provide such a significant benefit.133 New efforts are underway to explore the use of these agents in other clinical indications, and new radioconjugates are being examined in lymphoma and leukemia.112,134–138, [134], [135], [136], [137], [138]

The application of RAIT to other tumors is considerably more challenging. The higher radioresistance of solid tumors most certainly is the primary reason why RAIT has not been as successful for these tumors, since the targeting of a variety of solid tumors is as good, if not better, than that seen in lymphoma. Despite efforts to increase the administered radiation dose by using bone marrow or peripheral stem cell support, and even by combining high-dose radioimmunotherapy with chemotherapy, clinically significant antitumor responses in solid tumors remain elusive.108 A Phase III trial in lung cancer has indicated some success in advanced disease, but for the most part, as first emphasized in animal model testing, RAIT is more likely to succeed when the disease burden is minimal or when used as an adjuvant treatment.139–141, [140], [141] Early clinical studies appear to corroborate these preclinical findings, at least in colorectal cancer, where RAIT post salvage resection of colorectal liver metastases indicated a doubling of the survival time compared with historical or contemporaneous controls.142 Additionally, clinical studies are applying radiolabeled antibodies for intracompartmental treatments, such as intracranial and intraperitoneal therapies, where it may be possible to increase the accessibility and amount of antibody targeted to tumors in these regional areas.143–145, [144], [145] Preclinical studies have shown that nontherapeutic doses of chemotherapy can enhance the effects of RAIT, while other studies have shown that relatively small doses of radiolabeled antibodies can enhance the therapeutic activity of a standard chemotherapy regimen.146–151, [147], [148], [149], [150], [151] The reduced hematological toxicity associated with pretargeting approaches should allow radioconjugates to be combined more readily with cytotoxic drugs.152,153, [153] In addition, combinations with unconjugated antibodies, such as cetuximab that can enhance the tumor's radiosensitivity, may be another option for treating EGFR-positive tumors.154 Thus, while challenges remain for antibody-targeted radionuclides in solid tumors, preclinical and initial clinical studies are encouraging.

Drug Immunoconjugates

In the late 1950s, Mathé, et al. linked methotrexate to the globulin fraction of a hamster antiserum directed against the mouse leukemia L1210 cell line to protect mice from subsequent inoculation with L1210 cells, providing the first evidence that antibodies could be used to target drugs.155 As with radioconjugates, clinical success was first achieved in a hematological malignancy, with the FDA approving in 2000, gemtuzumab ozogamicin (Mylotarg; Wyeth Ayerst) for the treatment of relapsed acute myelocytic leukemia in adults (≥60 years of age).156 Gemtuzumab ozogamicin is a conjugate of a humanized anti-CD33 IgG linked to colicheamicin, a potent antitumor agent isolated from a bacterium. The prospects of using it as a front-line treatment and expanding its indications to include pediatric cancer patients, and in combination with chemotherapy, are under evaluation.157–160, [158], [159], [160] Aside from the standard precautions for side effects associated with its infusion, other primary side effects include complications associated with severe hematological toxicity, mucositis, as well as hepatotoxicity (hyperbilirubinemia, elevated ALT, AST, and biliribin).

Conjugation of a drug to an antibody alters the drug's pharmacodynamics, essentially “detoxifying” it, and this has allowed drugs that otherwise would be too toxic for human use alone (ie, ultratoxic drugs) to be tested as antibody-drug conjugates. Current clinical trials with drug conjugates almost exclusively use drugs that are far more potent than most chemotherapeutic agents, and other highly potent agents also are under development.161–167, [162], [163], [164], [165], [166], [167]

The union of a biologic (antibody) and a drug (a chemical) must be made chemically, with the conjugate retaining the binding activity of the antibody, as well as the biological activity of the drug (Figure 4). Drugs may be coupled directly to an antibody or to inert carriers, such as dextrans or amino acid polymers, which have been used to increase the drug-substitution level of the conjugate.161,162, [162] Responses are dose-dependent, and therefore, optimizing the drug-antibody substitution level will improve the chances for success. However, a careful balance between maximizing the drug payload and maintaining favorable pharmacokinetic and biodistribution properties must be achieved.

Leukemias are a particularly attractive target for immunoconjugate therapy since the individual cells are readily available in the bloodstream and marrow. Drugs must get inside the cell to be active, and therefore, a target that is actively internalized would be more important than the target's relative abundance. For example, MAbs against CD74, which is found in low density on B-cells, monocytes, lymphomas, myelomas, and certain carcinomas, have been reported to be highly efficient carriers for drugs, toxins, and radionuclides because CD74 is readily recycled.168–171, [169], [170], [171] However, gemtuzumab ozogamicin is active even in CD33-negative cell lines because these cells are highly endocytic, and therefore, the conjugate can be internalized without specifically binding to the cell.172 When internalized, the drug must be liberated from the antibody to regain its activity. Separation of the drug from the antibody generally occurs in the lysosomes. Ineffective trafficking and drug separation inside the cell can have a profound impact on the potency of the conjugate. Often, drugs are coupled to antibody using linkages that can only be cleaved in the acidic milieu of the lysosomes.161,163, [163] There were hopes that antibody-drug conjugates might overcome drug resistance by bypassing the P-glycoprotein mechanism for extruding drugs.173 Unfortunately, this has not been realized, but one study has suggested that this might be possible under certain circumstances.174–176, [175], [176]

Pretargeting approaches also have been applied to drugs. Most often referred to as ADEPT (antibody-directed enzyme prodrug therapy), this strategy first targets an antibody-enzyme conjugate to the tumor.177 Once the conjugate is sufficiently cleared from the blood, a prodrug, which is not biologically active, is given. The prodrug is converted to an active form and released from the enzyme-conjugate. Enzymatic conversion of the prodrug continues, resulting in locally increased levels of the active drug. The ADEPT method has been tested extensively in preclinical models, as well as in early Phase I clinical studies, which initially identified the immunogenicity and clearance of the antibody-enzyme conjugate as obstacles, but preclinical studies suggest that these problems may be overcome in the near future.178–180, [179], [180]

While there are still a number of challenges to be met, new agents are being developed that will likely lead to expanded clinical evaluation of drug immunoconjugates.

Toxin Immunoconjugates

Except for denileukin diftitox (Ligand Pharmaceuticals), which is a modified diphtheria toxin coupled to interleukin-2 for the treatment of cutaneous T-cell lymphoma, no other immunotoxins have been approved by the FDA; however, there have been a number of clinical trials with a variety of toxins conjugated to antibodies.181–183, [182], [183]

Toxins are truly ultratoxic agents, requiring relatively few copies to kill the cell, but they face the same delivery issues as a drug conjugate. Immunotoxins have been produced primarily from toxins that are ribosomal inactivating proteins, interfering with the reading of mRNA and thereby disrupting protein synthesis.182 Most are natural proteins derived from plants, bacteria, or fungi, but RNases isolated from vertebrates are also being examined.184 Since toxins have their own means for binding to cells, the cell-binding portion must be separated from the active portion of the toxin to improve targeting specificity (Figure 4).185 As proteins, toxins are amenable to recombinant production as antibody- (or other biological targeting substance, such as interleukin-2) toxin fusion proteins.182,183, [183] However, toxins are foreign proteins, and therefore the formation of neutralizing antibodies is a concern for repeated use. The possible exception is RNase, which may be less immunogenic.186

Therapy of B-cell lymphoma using ricin A-chain conjugates prepared chemically with deglycosylated ricin A-chain and either an anti-CD19 or an anti-CD22 murine IgG was limited by the development of vascular leak syndrome (consisting of edema, tachycardia, dyspnea, weakness, and myalgia).187–189, [188], [189] Recent insights into the molecular structure of the active ricin A- chain have revealed a motif that is responsible for binding to endothelial cells, which could be an important determinant in the development of dose-limiting vascular leak syndrome.190

A recombinant anti-CD22 × Pseudomonas exotoxin has been highly effective in patients with hairy cell leukemia, while not being as active in NHL CLL.191 In hairy cell leukemia, clinical benefit (86% CR rate with a median duration of 36 months) was observed after a single cycle of conjugate treatment at a dose level of 40 μg/kg every other day × 3, with the most common toxicities being hypoalbuminemia, transaminase elevations, fatigue, and edema; a reversible hemolytic uremic syndrome requiring plasmapheresis also was observed in several patients. This conjugate's activity in hairy cell leukemia and with manageable toxicity is an exciting new development for immunotoxin conjugates.

Similar to the experience with other immunoconjugates, solid tumors remain a formidable challenge for therapy with immunotoxins. An immunotoxin prepared as a recombinant Pseudomonas exotoxin × anti-Lewis-Y antibody (BR96) was tested in 46 patients with Lewis-Y-positive tumors, with no objective responses reported.192 The dose of this conjugate was limited by gastrointestinal toxicity, likely because BR96 is cross-reactive with normal gastrointestinal epithelium.193

ECONOMIC CONSIDERATIONS

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References

One lesson learned from this review is that the new biological agents, particularly the unconjugated MAbs, are more effective when used in combination with other therapeutic agents, including perhaps other antibodies. Since not all patients are responsive, presumably because of differences in the receptors being targeted, molecular testing will become part of the paradigm of biological therapy to choose drugs on an individual patient basis.

But these considerations can have staggering financial implications. If the average monthly price is $4,800 for bevacizumab and $12,000 for cetuximab, combinations of these together with drugs in colorectal cancer treatment can range between $11,000 and $27,000 monthly, along with pharmacy and dispensing costs. Since these can be prescribed over several months, the costs can challenge the heathcare system and third-party payers, as cautioned recently by Wittes.194

CONCLUSIONS

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References

Antibodies and immunoconjugates are gaining a significant and expanding role in the therapy of cancer. Because patients generally tolerate antibody treatments with minimal side effects, compared with many other cancer treatment modalities, immunotherapy with antibodies represents an exciting opportunity for combining with standard modalities, such as chemotherapy, as well as combinations between diverse biological agents, including antibody combinations in NHL therapy and possibly cetuximab + bevacizumab (with chemotherapy) in metastatic colorectal cancer.195 As we learn more about how cancer and other diseased cells control their proliferation and spread, undoubtedly unconjugated antibodies will be used to disrupt these functions by targeting important sites or regulators of cell proliferation, metabolism, adhesion, migration, spread, and other properties of malignancy. The use of antibodies to target radionuclides, drugs, and toxins is expanding as the next generation of MAb-based products for cancer therapy. At least in the case of targeted radionuclides, clinical studies have shown that these immunoconjugates are more effective than immunotherapy with the antibody alone, which highlights the enhanced efficacy achieved when a cytotoxic agent is targeted by an antibody that is also active.

This review has summarized the strides made over the past 25 years for developing new, selective, therapeutic strategies based on the evolution of various antibody forms and an identification of new cellular targets. Molecular biology has been at the basis of developing this new generation of antigen-binding molecules. As new target molecules and receptors on tumor cells are identified in the future, the experiences gained with the use of current immunoconjugates will enable a more rapid translation to clinical evaluation and use when next-generation antibodies and immunoconjugates are developed.

References

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. THERAPY WITH UNCONJUGATED ANTIBODIES
  5. IMMUNOCONJUGATES
  6. ECONOMIC CONSIDERATIONS
  7. CONCLUSIONS
  8. References
  • 1
    von Behring E, Kitasato S. Dtsch. Med. Wochenschr 1890; 16: 11131114.
  • 2
    Hericourt J, Richet CH. ‘Physologie pathologique’ - de la serotherapie dans la traitement du cancer. Comptes Rendus Hebd Seanc Acad Sci (Paris) 1895; 120: 567569.
  • 3
    Himmelweit F. The Collected Papers of Paul Ehrlich. Vol. 3. London: Pergamon; 1960: 59.
  • 4
    Nadler LM, Stashenko P, Hardy R, et al. Serotherapy of a patient with a monoclonal antibody directed against a human lymphoma-associated antigen. Cancer Res 1980; 40: 31473154.
  • 5
    Miller RA, Maloney DG, Warnke R, Levy R. Treatment of B-cell lymphoma with monoclonal anti-idiotype antibody. N Engl J Med 1982; 306: 517522.
  • 6
    Foon KA, Schroff RW, Bunn PA, et al. Effects of monoclonal antibody therapy in patients with chronic lymphocytic leukemia. Blood 1984; 64: 10851093.
  • 7
    Goldenberg DM, DeLand F, Kim E, et al. Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning. N Engl J Med 1978; 298: 13841386.
  • 8
    Gaffar SA, Pant KD, Shochat D, et al. Experimental studies of tumor radioimmunodetection using antibody mixtures against carcinoembryonic antigen (CEA) and colon-specific antigen-p (CSAp). Int J Cancer 1981; 27: 101105.
  • 9
    DeNardo SJ, DeNardo GL, O'Grady LF, et al. Treatment of B cell malignancies with 131 I Lym-1 monoclonal antibodies. Int J Cancer Suppl 1988; 3: 96101.
  • 10
    Goldenberg DM, Horowitz JA, Sharkey RM, et al. Targeting, dosimetry, and radioimmunotherapy of B-cell lymphomas with iodine-131-labeled LL2 monoclonal antibody. J Clin Oncol 1991; 9: 548564.
  • 11
    Buchsbaum DJ, Wahl RL, Normolle DP, Kaminski MS. Therapy with unlabeled and 131 I-labeled pan-B-cell monoclonal antibodies in nude mice bearing Raji Burkitt's lymphoma xenografts. Cancer Res 1992; 52: 64766481.
  • 12
    Kaminski MS, Zasadny KR, Francis IR, et al. Radioimmunotherapy of B-cell lymphoma with 131 I-anti-B1 (anti-CD20) antibody. N Engl J Med 1993; 329: 459465.
  • 13
    Maloney DG, Liles TM, Czerwinski DK, et al. Phase I clinical trial using escalating single-dose infusion of chimeric anti-CD20 monoclonal antibody (IDEC-C2B8) in patients with recurrent B-cell lymphoma. Blood 1994; 84: 24572466.
  • 14
    Jazirehi AR, Bonavida B. Cellular and molecular signal transduction pathways modulated by rituximab (Rituxan, anti-CD20 mAb) in non-Hodgkin's lymphoma: implications in chemosensitization and therapeutic intervention. Oncogene 2005; 24: 21212143.
  • 15
    Zhang N, Khawli LA, Hu P, Epstein AL. Generation of rituximab polymer may cause hyper-cross-linking-induced apoptosis in non-Hodgkin's lymphomas. Clin Cancer Res 2005; 11: 59715980.
  • 16
    Ghobrial IM, Witzig TE, Adjei AA. Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin 2005; 55: 178194.
  • 17
    Bianco R, Daniele G, Ciardiello F, Tortora G. Monoclonal antibodies targeting the epidermal growth factor receptor. Curr Drug Targets 2005; 6: 275287.
  • 18
    Emens LA. Trastuzumab: targeted therapy for the management of HER-2/neu-overexpressing metastatic breast cancer. Am J Ther 2005; 12: 243253.
  • 19
    Czuczman M. CHOP plus rituximab chemoimmunotherapy of indolent B-cell lymphoma. Semin Oncol 1999( 5 Suppl 14): 8896.
  • 20
    Marty M, Cognetti F, Maraninchi D, et al. Randomized phase II trial of the efficacy and safety of trastuzumab combined with docetaxel in patients with human epidermal growth factor receptor 2-positive metastatic breast cancer administered as first-line treatment: the M77001 study group. J Clin Oncol 2005; 23: 42654274.
  • 21
    Raben D, Helfrich B, Chan DC, et al. The effects of cetuximab alone and in combination with radiation and/or chemotherapy in lung cancer. Clin Cancer Res 2005; 11: 795805.
  • 22
    Ferrara N, Hillan KJ, Novotny W. Bevacizumab (Avastin), a humanized anti-VEGF monoclonal antibody for cancer therapy. Biochem Biophys Res Commun 2005; 333: 328335.
  • 23
    Leach DR, Krummel MF, Allison JP. Enhancement of antitumor immunity by CTLA-4 blockade. Science 1996; 271: 17341736.
  • 24
    Kapadia D, Fong L. CTLA-4 blockade: autoimmunity as treatment. J Clin Oncol 2005; 23: 89268928.
  • 25
    Rutgeerts P, Van Assche G, Vermeire S. Review article: inflab therapy for inflammatory bowel disease—seven years on. Aliment Pharmacol Ther 2006; 23: 451463.
  • 26
    Cuppoletti A, Perez-Villa F, Vallejos I, Roig E. Experience with single-dose daclizumab in the prevention of acute rejection in heart transplantation. Transplant Proc 2005; 37: 40364038.
  • 27
    Liossis SN, Tsokos GC. Monoclonal antibodies and fusion proteins in medicine. J Allergy Clin Immunol 2005; 116: 721729.
  • 28
    Chatenoud L. Monoclonal antibody-based strategies in autoimmunity and transplantation. Methods Mol Med 2005; 109: 297328.
  • 29
    Chambers SA, Isenberg D. Anti-B cell therapy (rituximab) in the treatment of autoimmune diseases. Lupus 2005; 14: 210214.
  • 30
    Looney RJ. B cell-targeted therapy in diseases other than rheumatoid arthritis. J Rheumatol Suppl 2005; 73: 2528.
  • 31
    Kaufmann J, Wegener WA, Horak ID, et al. Initial clinical study of immunotherapy in SLE using epratuzumab (humanized anti-CD22 antibody). Arthritis Rheum 2004; 50: S447.
  • 32
    Ilantzis C, DeMarte L, Screaton RA, Stanners CP. Deregulated expression of the human tumor marker CEA and CEA family member CEACAM6 disrupts tissue architecture and blocks colonocyte differentiation. Neoplasia 2002; 4: 151163.
  • 33
    Blumenthal RD, Osorio L, Hayes MK, et al. Carcinoembryonic antigen antibody inhibits lung metastasis and augments chemotherapy in a human colonic carcinoma xenograft. Cancer Immunol Immunother 2005; 54: 315327.
  • 34
    Jain RK. Transport of molecules, particles, and cells in solid tumors. Annu Rev Biomed Eng 1999; 1: 241263.
  • 35
    Fujimori K, Covell DG, Fletcher JE, Weinstein JN. A modeling analysis of monoclonal antibody percolation through tumors: a binding-site barrier. J Nucl Med 1990; 31: 11911198.
  • 36
    Adams GP, Schier R, McCall AM, et al. High affinity restricts the localization and tumor penetration of single-chain fv antibody molecules. Cancer Res 2001; 61: 47504755.
  • 37
    Blumenthal RD, Fand I, Sharkey RM, et al. The effect of antibody protein dose on the uniformity of tumor distribution of radioantibodies: an autoradiographic study. Cancer Immunol Immunother 1991; 33: 351358.
  • 38
    Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256: 495497.
  • 39
    Sears HF, Herlyn D, Steplewski Z, Koprowski H. Phase II clinical trial of a murine monoclonal antibody cytotoxic for gastrointestinal adenocarcinoma. Cancer Res 1985; 45: 59105913.
  • 40
    Houghton AN, Mintzer D, Cordon-Cardo C, et al. Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: A phase I trial in patients with malignant melanoma. Proc Natl Acad Sci, USA 1985; 82: 12421246.
  • 41
    Goodman GE, Beaumier P, Hellstrom I, et al. Pilot trial of murine monoclonal antibodies in patients with advanced melanoma. J Clin Oncol 1985; 3: 340352.
  • 42
    Waldmann H, Hale G. CAMPATH: from concept to clinic. Philos Trans R Soc Lond B Biol Sci 2005; 360: 17071711.
  • 43
    Morrison SL, Johnson MJ, Herzenberg LA, Oi VT. Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains. Proc Natl Acad Sci USA 1984; 81: 68516855.
  • 44
    Jones PT, Dear PH, Foote J, et al. Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature 1986; 321: 522525.
  • 45
    Qu Z, Griffiths GL, Wegener WA, et al. Development of humanized antibodies as cancer therapeutics. Methods 2005; 36: 8495.
  • 46
    Moroney S, Plückthun A. Modern antibody technology: The impact on drug development. In: KnäbleinJ, ed. Modern Biopharmaceuticals. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co KGaA; 2005; 11471186.
  • 47
    Weng WK, Levy R. Two immunoglobulin G fragment C receptor polymorphisms independently predict response to rituximab in patients with follicular lymphoma. J Clin Oncol 2003; 21: 39403947.
  • 48
    McLaughlin P, Grillo-Lopez AJ, Link BK, et al. Rituximab chimeric anti-CD20 monoclonal antibody therapy for relapsed indolent lymphoma: half of patients respond to a four-dose treatment program. J Clin Oncol 1998; 16: 28252833.
  • 49
    Available at http://www.rituxan.com.
  • 50
    Davis TA, Grillo-López AJ, White CA, et al. Rituximab anti-CD20 monoclonal antibody therapy in non-Hodgkin's lymphoma: safety and efficacy of re-treatment. J Clin Oncol 2000; 18: 31353143.
  • 51
    Hainsworth JD, Litchy S, Shaffer DW, et al. Maximizing therapeutic benefit of rituximab: maintenance therapy versus re-treatment at progression in patients with indolent non-Hodgkin's lymphoma—a randomized phase II trial of the Minnie Pearl Cancer Res Network. J Clin Oncol 2005; 23: 10881095.
  • 52
    Coiffier B. First-line treatment of follicular lymphoma in the era of monoclonal antibodies. Clin Adv Hematol Oncol 2005; 3: 484505.
  • 53
    Coiffier B. Rituximab in diffuse large B-cell lymphoma. Clin Adv Hematol Oncol 2004; 2: 156157.
  • 54
    Byrd JC, Murphy T, Howard RS, et al. Rituximab using a thrice weekly dosing schedule in B-cell chronic lymphocytic leukemia and small lymphocytic lymphoma demonstrates clinical activity and acceptable toxicity. J Clin Oncol 2001; 19: 21532164.
  • 55
    O'Brien SM, Kantarjian H, Thomas DA, et al. Rituximab dose-escalation trial in chronic lymphocytic leukemia. J Clin Oncol 2001; 19: 21652170.
  • 56
    Leonard JP, Coleman M, Ketas JC, et al. Epratuzumab, a humanized anti-CD22 antibody, in aggressive non-Hodgkin's lymphoma: phase I/II clinical trial results. Clin Cancer Res 2004; 10: 53275334.
  • 57
    Leonard JP, Coleman M, Ketas J, et al. Combination antibody therapy with epratuzumab and rituximab in relapsed or refractory non-Hodgkin's lymphoma. J Clin Oncol 2005; 23: 50445051.
  • 58
    Younes A, Hariharan K, Allen RS, Leigh BR. Initial trials of anti-CD80 monoclonal antibody (Galiximab) therapy for patients with relapsed or refractory follicular lymphoma. Clin Lymphoma 2003; 3: 257259.
  • 59
    Czuczman MS, Thall A, Witzig TE, et al. Phase I/II study of galiximab, an anti-CD80 antibody, for relapsed or refractory follicular lymphoma. J Clin Oncol 2005; 23: 43904398.
  • 60
    Reff ME, Carner K, Chambers KS, et al. Depletion of B cells in vivo by a chimeric mouse human monoclonal antibody to CD20. Blood 1994; 83: 435445.
  • 61
    Golay J, Lazzari M, Facchinetti V, et al. CD20 levels determine the in vitro susceptibility to rituximab and complement of B-cell chronic lymphocytic leukemia: further regulation by CD55 and CD59. Blood 2001; 98: 33833389.
  • 62
    Shan D, Ledbetter JA, Press OW. Apoptosis of malignant human B cells by ligation of CD20 with monoclonal antibodies. Blood 1998; 91: 16441652.
  • 63
    Golay J, Zaffaroni L, Vaccari T, et al. Biologic response of B lymphoma cells to anti-CD20 monoclonal antibody rituximab in vitro: CD55 and CD59 regulate complement-mediated cell lysis. Blood 2000; 95: 39003908.
  • 64
    Treon SP, Mitsiades C, Mitsiades N, et al. Tumor cell expression of CD59 is associated with resistance to CD20 serotherapy in patients with B-cell malignancies. J Immunother 2001; 24: 263271.
  • 65
    Weng WK, Levy R. Expression of complement inhibitors CD46, CD55, and CD59 on tumor cells does not predict clinical outcome after rituximab treatment in follicular non-Hodgkin lymphoma. Blood 2001; 98: 13521357.
  • 66
    Manches O, Lui G, Chaperot L, et al. In vitro mechanisms of action of rituximab on primary non-Hodgkin lymphomas. Blood 2003; 101: 949954.
  • 67
    Uchida J, Hamaguchi Y, Oliver JA, et al. The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy. J Exp Med 2004; 199: 16591669.
  • 68
    Hernandez-Ilizaliturri FJ, Jupudy V, Ostberg J, et al. Neutrophils contribute to the biological antitumor activity of rituximab in a non-Hodgkin's lymphoma severe combined immunodeficiency mouse model. Clin Cancer Res 2003; 9: 58665873.
  • 69
    Presta LG. Engineering antibodies for therapy. Curr Pharm Biotechnol 2002; 3: 237256.
  • 70
    Shields RL, Lai J, Keck R, et al. Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. J Biol Chem 2002; 277: 26733267340.
  • 71
    Hodoniczky J, Zheng YZ, James DC. Control of recombinant monoclonal antibody effector functions by Fc N-glycan remodeling in vitro. Biotechnol Prog 2005; 21: 16441652.
  • 72
    Cartron G, Dacheux L, Salles G, et al. Therapeutic activity of humanized anti-CD20 monoclonal antibody and polymorphism in IgG Fc receptor FcgammaRIIIa gene. Blood 2002; 99: 754758.
  • 73
    Kakinoki Y, Kubota H, Yamamoto Y. CD64 surface expression on neutrophils and monocytes is significantly up-regulated after stimulation with granulocyte colony-stimulating factor during CHOP chemotherapy for patients with non-Hodgkin's lymphoma. Int J Hematol 2004; 79: 5562.
  • 74
    Parihar R, Dierksheide J, Hu Y, Carson WE. IL-12 enhances the natural killer cell cytokine response to Ab-coated tumor cells. J Clin Invest 2002; 110: 983992.
  • 75
    Ansell SM, Witzig TE, Kurtin PJ, et al. Phase 1 study of interleukin-12 in combination with rituximab in patients with B-cell non-Hodgkin lymphoma. Blood 2002; 99: 6774.
  • 76
    Presta LG, Shields RL, Namenuk AK, et al. Engineering therapeutic antibodies for improved function. Biochem Soc Trans 2002; 30: 487490.
  • 77
    Vaccaro C, Zhou J, Ober RJ, Ward ES. Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol 2005; 23: 12831288.
  • 78
    Idusogie EE, Wong PY, Presta LG, et al. Engineered antibodies with increased activity to recruit complement. J Immunol 2001; 166: 25712575.
  • 79
    Stockmeyer B, Elsasser D, Dechant M, et al. Mechanisms of G-CSF- or GM-CSF-stimulated tumor cell killing by Fc receptor-directed bispecific antibodies. J Immunol Methods 2001; 248: 103111.
  • 80
    Bevaart L, Jansen MJ, van Vugt MJ, et al. The high-affinity IgG receptor, FcgammaRI, plays a central role in antibody therapy of experimental melanoma. Cancer Res 2006; 66: 12611264.
  • 81
    Slamon DJ, Leyland-Jones B, Shak S, et al. Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N Engl J Med 2001; 344: 783792.
  • 82
    Piccart-Gebhart MJ, Procter M, Leyland-Jones B, et al. Trastuzumab after adjuvant chemotherapy in HER2-positive breast cancer. N Engl J Med 2005; 353: 16591672.
  • 83
    Romond EH, Perez EA, Bryant J, et al. Trastuzumab plus adjuvant chemotherapy for operable HER2-positive breast cancer. N Engl J Med 2005; 353: 16731684.
  • 84
    Izumi Y, Xu L, di Tomaso E, et al. Tumour biology: herceptin acts as an anti-angiogenic cocktail. Nature 2002; 416: 279280.
  • 85
    Gennari R, Menard S, Fagnoni F, et al. Pilot study of the mechanism of action of preoperative trastuzumab in patients with primary operable breast tumors overexpressing HER2. Clin Cancer Res 2004; 10: 56505655.
  • 86
    Warburton C, Dragowska WH, Gelmon K, et al. Treatment of HER-2/neu overexpressing breast cancer xenograft models with trastuzumab (Herceptin) and gefitinib (ZD1839): drug combination effects on tumor growth, HER-2/neu and epidermal growth factor receptor expression, and viable hypoxic cell fraction. Clin Cancer Res 2004; 10: 25122524.
  • 87
    Negro A, Brar BK, Lee KF. Essential roles of Her2/erbB2 in cardiac development and function. Recent Prog Horm Res 2004; 59: 112.
  • 88
    Garratt AN, Ozcelik C, Birchmeier C. ErbB2 pathways in heart and neural diseases. Trends Cardiovasc Med 2003; 13: 8086.
  • 89
    Ewer MS, Vooletich MT, Durand JB, et al. Reversibility of trastuzumab-related cardiotoxicity: new insights based on clinical course and response to medical treatment. J Clin Oncol 2005; 23: 78207826.
  • 90
    Tan-Chiu E, Yothers G, Romond E, et al. Assessment of cardiac dysfunction in a randomized trial comparing doxorubicin and cyclophosphamide followed by paclitaxel, with or without trastuzumab as adjuvant therapy in node-positive, human epidermal growth factor receptor 2-overexpressing breast cancer: NSABP B-31. J Clin Oncol 2005; 23: 78117819.
  • 91
    Normanno N, Bianco C, De Luca A, et al. Target-based agents against ErbB receptors and their ligands: a novel approach to cancer treatment. Endocr Relat Cancer 2003; 10: 121.
  • 92
    Guan H, Jia SF, Zhou Z, et al. Herceptin down-regulates HER-2/neu and vascular endothelial growth factor expression and enhances taxol-induced cytotoxicity of human Ewing's sarcoma cells in vitro and in vivo. Clin Cancer Res 2005; 11: 20082017.
  • 93
    Bonner JA, Harari PM, Giralt J, et al. Radiotherapy plus cetuximab for squamous-cell carcinoma of the head and neck. N Engl J Med 2006; 354: 567578.
  • 94
    Perez-Soler R, Saltz L. Cutaneous adverse effects with HER1/EGFR-targeted agents: is there a silver lining? J Clin Oncol 2005; 23: 52355246.
  • 95
    Kanai T, Konno H, Tanaka T, et al. Anti-tumor and anti-metastatic effects of human-vascular-endothelial-growth-factor-neutralizing antibody on human colon and gastric carcinoma xenotransplanted orthotopically into nude mice. Int J Cancer 1998; 77: 933936.
  • 96
    Gorski DH, Beckett MA, Jaskowiak NT, et al. Blockage of the vascular endothelial growth factor stress response increases the antitumor effects of ionizing radiation. Cancer Res 1999; 59: 33743378.
  • 97
    de Gramont A, Van Cutsem E. Investigating the potential of bevacizumab in other indications: metastatic renal cell, non-small cell lung, pancreatic and breast cancer. Oncol 2005; 69: 4656.
  • 98
    D'Adamo DR, Anderson SE, Albritton K, et al. Phase II study of doxorubicin and bevacizumab for patients with metastatic soft-tissue sarcomas. J Clin Oncol 2005; 23: 71357142.
  • 99
    Bruns I, Fox F, Reinecke P, et al. Complete remission in a patient with relapsed angioimmunoblastic T-cell lymphoma following treatment with bevacizumab. Leukemia 2005; 19: 19931995.
  • 100
    Gordon MS, Cunningham D. Managing patients treated with bevacizumab combination therapy. Oncology 2005; 69: 2533.
  • 101
    Witzig TE, Gordon LI, Cabanillas F, et al. Randomized controlled trial of yttrium-90-labeled ibritumomab tiuxetan radioimmunotherapy versus rituximab immunotherapy for patients with relapsed or refractory low-grade, follicular, or transformed B-cell non-Hodgkin's lymphoma. J Clin Oncol 2002; 20: 24532463.
  • 102
    Davis TA, Kaminski MS, Leonard JP, et al. The radioisotope contributes significantly to the activity of radioimmunotherapy. Clin Cancer Res 2004; 10: 77927798.
  • 103
    Silverstein AM. Labeled antigens and antibodies: the evolution of magic markers and magic bullets. Nat Immunol 2004; 5: 12111217.
  • 104
    Goldenberg DM. Perspectives on oncologic imaging with radiolabeled antibodies. Cancer 1997; 80: 24312435.
  • 105
    Larson SM, Pentlow KS, Volkow ND, et al. PET scanning of iodine-124-3F9 as an approach to tumor dosimetry during treatment planning for radioimmunotherapy in a child with neuroblastoma. J Nucl Med 1992; 33: 20202023.
  • 106
    Wong JY, Chu DZ, Williams LE, et al. Pilot trial evaluating an 123 I-labeled 80-kilodalton engineered anticarcinoembryonic antigen antibody fragment (cT84. 66 minibody) in patients with colorectal cancer. Clin Cancer Res 2004; 10: 50145021.
  • 107
    McBride WJ, Zanzonico P, Sharkey RM, et al. Bispecific antibody pretargeting PET (ImmunoPET) with an 124I-labeled hapten-peptide. J Nucl Med. In press, 2006.
  • 108
    Sharkey RM, Goldenberg DM. Perspectives on cancer therapy with radiolabeled monoclonal antibodies. J Nucl Med 2005; 46: 115s127s.
  • 109
    Roberson PL, Buchsbaum DJ. Reconciliation of tumor dose response to external beam radiotherapy versus radioimmunotherapy with 131 Iodine-labeled antibody for a colon cancer model. Cancer Res 1995; 55: 5811s5816s.
  • 110
    Hernandez MC, Knox SJ. Radiobiology of radioimmunotherapy with 90 Y ibritumomab tiuxetan (Zevalin). Semin Oncol 2003; 30: 610.
  • 111
    Kassis AI, Adelstein SJ. Radiobiologic principals in radionuclide therapy. J Nucl Med 2005; 46: 4s12s.
  • 112
    Kotzerke J, Bunjes D, Scheinberg DA. Radioimmunoconjugates in acute leukemia treatment: the future is radiant. Bone Marrow Transplant 2005; 36: 10211026.
  • 113
    Michel RB, Brechbiel MW, Mattes MJ. A comparison of 4 radionuclides conjugated to antibodies for single-cell kill. J Nucl Med 2003; 44: 632640.
  • 114
    Olafsen T, Kenanova VE, Sundaresan G, et al. Optimizing radiolabeled engineered anti-p185HER2 antibody fragments for in vivo imaging. Cancer Res 2005; 65: 59075916.
  • 115
    Kenanova V, Olafsen T, Crow DM, et al. Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments. Cancer Res 2005; 65: 622631.
  • 116
    Behr TM, Goldenberg DM, Becker W. Reducing the renal uptake of radiolabeled antibody fragments and peptides for diagnosis and therapy: present status, future prospects and limitations. Eur J Nucl Med 1998; 25: 201212.
  • 117
    Sharkey RM, Karacay H, Cardillo TM, et al. Improving the delivery of radionuclides for imaging and therapy of cancer using pretargeting methods. Clin Cancer Res 2005; 11: 7109s7121s.
  • 118
    Sharkey RM, Cardillo TM, Rossi EA, et al. Signal amplification in molecular imaging by pretargeting a multivalent, bispecific antibody. Nat Med 2005; 11: 12501255.
  • 119
    Karacay H, Brard PY, Sharkey RM, et al. Therapeutic advantage of pretargeted radioimmunotherapy using a recombinant bispecific antibody in a human colon cancer xenograft. Clin Cancer Res 2005; 11: 78797885.
  • 120
    Rossi EA, Goldenberg DM, Cardillo TM, et al. Stably tethered multifunctional structures of defined composition made by the dock and lock method for use in cancer targeting. Proc Natl Acad Sci USA 2006; 103: 68416846.
  • 121
    Lin Y, Pagel JM, Axworthy D, et al. A genetically engineered anti-CD45 single-chain antibody-streptavidin fusion protein for pretargeted radioimmunotherapy of hematologic malignancies. Cancer Res 2006; 66: 38843892.
  • 122
    Goldenberg DM, Sharkey RM, Paganelli G, et al. Antibody pretargeting advances cancer radioimmunodetection and radioimmunotherapy. J Clin Oncol 2006; 24: 823834.
  • 123
    Shen S, Forero A, LoBuglio AF, et al. Patient-specific dosimetry of pretargeted radioimmunotherapy using CC49 fusion protein in patients with gastrointestinal malignancies. J Nucl Med 2005; 46: 642651.
  • 124
    Chatal J-F, Campion L, Kraeber-Bodéré F, et al. Calcitonin doubling time predicts survival improvement in medullary thyroid carcinoma patients given pretargeted CEA radioimmunotherapy. J Clin Oncol 2006; 24: 17051711.
  • 125
    Sharkey RM, Burton J, Goldenberg DM. Radioimmunotherapy of non-Hodgkin's lymphoma: a critical appraisal. Expert Rev Clin Immunol 2005; 1: 4762.
  • 126
    Gordon LI, Molina A, Witzig T, et al. Durable responses after ibritumomab tiuxetan radioimmunotherapy for CD20+ B-cell lymphoma: long-term follow-up of a phase 1/2 study. Blood 2004; 103: 44294431.
  • 127
    Wiseman GA, Witzig TE. Yttrium-90 (90 Y) ibritumomab tiuxetan (Zevalin) induces long-term durable responses in patients with relapsed or refractory B-cell non-Hodgkin's lymphoma. Cancer Biother Radiopharm 2005; 20: 185188.
  • 128
    Fisher RI, Kaminski MS, Wahl RL, et al. Tositumomab and iodine-131 tositumomab produces durable complete remissions in a subset of heavily pretreated patients with low-grade and transformed non-Hodgkin's lymphomas. J Clin Oncol 2005; 23: 75657573.
  • 129
    Kaminski MS, Tuck M, Estes J, et al. 131 I-tositumomab therapy as initial treatment for follicular lymphoma. N Engl J Med 2005; 352: 441449.
  • 130
    Sweetenham JW, Dicke K, Arcaroli J et al. Efficacy and safety of Yttrium 90 (90 Y) ibritumomab tiuxetan (Zevalin®) therapy with rituximab maintenance in patients with untreated low-grade follicular lymphoma. Blood 2004; 104: (abstract 2633).
  • 131
    Kaminski MS, Radford JA, Gregory SA, et al. Re-treatment with I-131 tositumomab in patients with non-Hodgkin's lymphoma who had previously responded to I-131 tositumomab. J Clin Oncol 2005; 23: 79857993.
  • 132
    Ansell SM, Ristow KM, Habermann TM, et al. Subsequent chemotherapy regimens are well tolerated after radioimmunotherapy with yttrium-90 ibritumomab tiuxetan for non-Hodgkin's lymphoma. J Clin Oncol 2002; 20: 38853890.
  • 133
    Connors JM. Radioimmunotherapy-hot new treatment for lymphoma. N Engl J Med 2005; 352: 496498.
  • 134
    Gopal AK, Gooley TA, Maloney DG, et al. High-dose radioimmunotherapy versus conventional high-dose therapy and autologous hematopoietic stem cell transplantation for relapsed follicular non-Hodgkin lymphoma: a multivariable cohort analysis. Blood 2003; 102: 23512357.
  • 135
    Vose JM, Bierman PJ, Enke C, et al. Phase I trial of iodine-131 tositumomab with high-dose chemotherapy and autologous stem-cell transplantation for relapsed non-Hodgkin's lymphoma. J Clin Oncol 2005; 23: 461467.
  • 136
    Leonard JP, Coleman M, Kostakoglu L, et al. Abbreviated chemotherapy with fludarabine followed by tositumomab and iodine I 131 tositumomab for untreated follicular lymphoma. J Clin Oncol 2005; 23: 56965704.
  • 137
    Sharkey RM, Brenner A, Burton J, et al. Radioimmunotherapy of non-Hodgkin's lymphoma with 90 Y-DOTA humanized anti-CD22 IgG (90 Y-epratuzumab): do tumor targeting and dosimetry predict therapeutic response? J Nucl Med 2003; 44: 20002018.
  • 138
    Linden O, Hindorf C, Cavallin-Stahl E, et al. Dose-fractionated radioimmunotherapy in non-Hodgkin's lymphoma using DOTA-conjugated, 90 Y-radiolabeled, humanized anti-CD22 monoclonal antibody, epratuzumab. Clin Cancer Res 2005; 11: 52155222.
  • 139
    Chen S, Yu L, Jiang C, et al. Pivotal study of iodine-131-labeled chimeric tumor necrosis treatment radioimmunotherapy in patients with advanced lung cancer. J Clin Oncol 2005; 23: 15381547.
  • 140
    Sharkey RM, Pykett MJ, Siegel JA, et al. Radioimmunotherapy of the GW-39 human colonic tumor xenograft with 131 I-labeled murine monoclonal antibody to carcinoembryonic antigen. Cancer Res 1987; 47: 56725677.
  • 141
    Blumenthal RD, Sharkey RM, Haywood L, et al. Targeted therapy of athymic mice bearing GW-39 human colonic cancer micrometastases with 131 I-labeled monoclonal antibodies. Cancer Res 1992; 52: 60366044.
  • 142
    Liersch T, Meller J, Kulle B, et al. Phase II trial of carcinoembryonic antigen radioimmunotherapy with 131 I-labetuzumab after salvage resection of colorectal metastases in the liver: five-year safety and efficacy results. J Clin Oncol 2005; 23: 67636770.
  • 143
    Reardon DA, Akabani G, Coleman RE, et al. Salvage radioimmunotherapy with murine iodine-131-labeled antitenascin monoclonal antibody 81C6 for patients with recurrent primary and metastatic malignant brain tumors: phase II study results. J Clin Oncol 2006; 24: 115122.
  • 144
    Alvarez RD, Huh WK, Khazaeli MB, et al. A phase I study of combined modality 90 Yttrium-CC49 intraperitoneal radioimmunotherapy for ovarian cancer. Clin Cancer Res 2002; 8: 28062811.
  • 145
    Mahe MA, Fumoleau P, Fabbro M, et al. A phase II study of intraperitoneal radioimmunotherapy with iodine-131-labeled monoclonal antibody OC-125 in patients with residual ovarian carcinoma. Clin Cancer Res 1999; 5: 3249s3253s.
  • 146
    DeNardo SJ, Kukis DL, Kroger LA, et al. Synergy of taxol and radioimmunotherapy with yttrium-90-labeled chimeric L6 antibody: efficacy and toxicity in breast cancer xenografts. Proc Natl Acad Sci USA 1997; 94: 40004004.
  • 147
    Tschmelitsch J, Barendswaard E, Williams C, et al. Enhanced antitumor activity of combination radioimmunotherapy (131 I-labeled monoclonal antibody A33) with chemotherapy (fluorouracil). Cancer Res 1997; 57: 21812186.
  • 148
    Clarke K, Lee FT, Brechbiel MW, et al. Therapeutic efficacy of anti-Lewis(y) humanized 3S193 radioimmunotherapy in a breast cancer model: enhanced activity when combined with taxol chemotherapy. Clin Cancer Res 2000; 6: 36213628.
  • 149
    Burke PA, DeNardo SJ, Miers LA, et al. Combined modality radioimmunotherapy. Promise and peril. Cancer 2002; 94( suppl): 13201331.
  • 150
    Gold DV, Modrak DE, Schutsky K, Cardillo TM. Combined 90 yttrium-DOTA-labeled PAM4 antibody radioimmunotherapy and gemcitabine radiosensitization for the treatment of a human pancreatic cancer xenograft. Int J Cancer 2004; 109: 618626.
  • 151
    Gold DV, Schutsky K, Modrak D, Cardillo TM. Low-dose radioimmunotherapy (90 Y-PAM4) combined with gemcitabine for the treatment of experimental pancreatic cancer. Clin Cancer Res 2003; 9: 3929S3937S.
  • 152
    Graves SS, Dearstyne E, Lin Y, et al. Combination therapy with pretarget CC49 radioimmunotherapy and gemcitabine prolongs tumor doubling time in a murine xenograft model of colon cancer more effectively than either monotherapy. Clin Cancer Res 2003; 9: 37123721.
  • 153
    Kraeber-Bodere F, Sai-Maurel C, Campion L, et al. Enhanced antitumor activity of combined pretargeted radioimmunotherapy and paclitaxel in medullary thyroid cancer xenograft. Mol Cancer Ther 2002; 1: 267274.
  • 154
    Baumann M, Krause M. Targeting the epidermal growth factor receptor in radiotherapy: radiobiological mechanisms, preclinical and clinical results. Radiother Oncol 2004; 72: 257266.
  • 155
    Mathé G, Loc TB, Bernard J. Effet sur la leucémie 1210 de la souris d'une combinaison par diazotation d'A-méthoptérine et de γ-globulines de hamsters porteurs de cette leucémie par hétérogreffe. C R Acad Sci (Paris) 1958; 246: 16261628.
  • 156
    Bross PF, Beitz J, Chen G, et al. Approval summary: gemtuzumab ozogamicin in relapsed acute myeloid leukemia. Clin Cancer Res 2001; 7: 14901496.
  • 157
    Larson RA, Sievers EL, Stadtmauer EA, et al. Final report of the efficacy and safety of gemtuzumab ozogamicin (Mylotarg) in patients with CD33-positive acute myeloid leukemia in first recurrence. Cancer 2005; 104: 14421452.
  • 158
    Chevallier P, Roland V, Mahe B, et al. Administration of mylotarg 4 days after beginning of a chemotherapy including intermediate-dose aracytin and mitoxantrone (MIDAM regimen) produces a high rate of complete hematologic remission in patients with CD33+ primary resistant or relapsed acute myeloid leukemia. Leuk Res 2005; 29: 10031007.
  • 159
    Amadori S, Suciu S, Stasi R, et al. Gemtuzumab ozogamicin (Mylotarg®) as single-agent treatment for frail patients 61 years of age and older with acute myeloid leukemia: final results of AML-15B, a phase 2 study of the European Organisation for Research and Treatment of Cancer and Gruppo Italiano Malattie Ematologiche dell'Adulto Leukemia Groups. Leukemia 2005; 19: 17681773.
  • 160
    Arceci RJ, Sande J, Lange B, et al. Safety and efficacy of gemtuzumab ozogamicin in pediatric patients with advanced CD33+ acute myeloid leukemia. Blood 2005; 106: 11831188.
  • 161
    Wu AM, Senter PD. Arming antibodies: prospects and challenges for immunoconjugates. Nature Biotechnol 2005; 23: 11371146.
  • 162
    Chen J, Jaracz S, Zhao X, et al. Antibody-cytotoxic agent conjugates for cancer therapy. Expert Opin Drug Deliv 2005; 2: 873890.
  • 163
    Govindan SV, Griffiths GL, Hansen HJ, et al. Cancer therapy with radiolabeled and drug/toxin-conjugated antibodies. Technol Cancer Res Treat 2005; 4: 375391.
  • 164
    Smith SV. Technology evaluation: cantuzumab mertansine, ImmunoGen. Curr Opin Mol Ther 2004; 6: 666674.
  • 165
    Law CL, Cerveny CG, Gordon KA, et al. Efficient elimination of B-lineage lymphomas by anti-CD20-auristatin conjugates. Clin Cancer Res 2004; 10: 78427851.
  • 166
    Torgov MY, Alley SC, Cerveny CG, et al. Generation of an intensely potent anthracycline by a monoclonal antibody-beta-galactosidase conjugate. Bioconjug Chem 2005; 16: 717721.
  • 167
    Hamann PR, Hinman LM, Beyer CF, et al. A calicheamicin conjugate with a fully humanized anti-MUC1 antibody shows potent antitumor effects in breast and ovarian tumor xenografts. Bioconjug Chem 2005; 16: 354360.
  • 168
    Burton JD, Ely S, Reddy PK, et al. CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res 2004; 10: 66066611.
  • 169
    Griffiths GL, Mattes MJ, Stein R, et al. Cure of SCID mice bearing human B-lymphoma xenografts by an anti-CD74 antibody-anthracycline drug conjugate. Clin Cancer Res 2003; 9: 65676571.
  • 170
    Sapra P, Stein R, Pickett J, et al. Anti-CD74 antibody-doxorubicin conjugate, IMMU-110, in a human multiple myeloma xenograft and in monkeys. Clin Cancer Res 2005; 11: 52575264.
  • 171
    Chang CH, Sapra P, Vanama SS, et al. Effective therapy of human lymphoma xenografts with a novel recombinant ribonuclease/anti-CD74 humanized IgG4 antibody immunotoxin. Blood 2005; 106: 43084314.
  • 172
    Jedema I, Barge RM, van der Velden VH, et al. Internalization and cell cycle-dependent killing of leukemic cells by gemtuzumab ozogamicin: rationale for efficacy in CD33-negative malignancies with endocytic capacity. Leukemia 2004; 18: 316325.
  • 173
    Leslie EM, Deeley RG, Cole SP. Multidrug resistance proteins: role of P-glycoprotein, MRP1, MRP2, and BCRP (ABCG2) in tissue defense. Toxicol Appl Pharmacol 2005; 204: 216237.
  • 174
    Naito K, Takeshita A, Shigeno K, et al. Calicheamicin conjugated humanized anti-CD33 monoclonal antibody (gemtuzumab zogamicin CMA-676) shows cytocidal effects on CD33-positive leukemia cell lines, but is inactive on P-glycoprotein-expressing sublines. Leukemia 2000; 14: 14361443.
  • 175
    Hamann PR, Hinman LM, Beyer CF, et al. An anti-MUC1 antibody-calicheamicin conjugate for treatment of solid tumors. Choice of linker and overcoming drug resistance. Bioconjug Chem 2005; 16: 346353.
  • 176
    Hamann PR, Hinman LM, Beyer CF, et al. An anti-CD33 antibody-calicheamicin conjugate for treatment of acute myeloid leukemia. Choice of linker. Bioconjug Chem 2002; 13: 4046.
  • 177
    Sharma SK, Bagshawe KD, Begent RH. Advances in antibody-directed enzyme prodrug therapy. Curr Opin Investig Drugs 2005; 6: 611615.
  • 178
    Francis RJ, Sharma SK, Springer C, et al. A phase I trial of antibody directed enzyme prodrug therapy (ADEPT) in patients with advanced colorectal carcinoma or other CEA producing tumours. Br J Cancer 2002; 87: 600607.
  • 179
    Mayer A, Sharma SK, Tolner B, et al. Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT). Br J Cancer 2004; 90: 24022410.
  • 180
    Cortez-Retamozo V, Backmann N, Senter PD, et al. Efficient cancer therapy with a nanobody-based conjugate. Cancer Res 2004; 64: 28532857.
  • 181
    Eklund JW, Kuzel TM. Denileukin diftitox: a concise clinical review. Expert Rev Anticancer Ther 2005; 5: 3338.
  • 182
    Frankel AE, Kreitman RJ, Sausville EA. Targeted toxins. Clin Cancer Res 2000; 6: 326334.
  • 183
    Pastan I. Immunotoxins containing Pseudomonas exotoxin A: a short history. Cancer Immunol Immunother 2003; 52: 338341.
  • 184
    Newton DL, Hansen HJ, Mikulski SM, et al. Potent and specific antitumor effects of an anti-CD22-targeted cytotoxic ribonuclease: potential for the treatment of non-Hodgkin lymphoma. Blood 2001; 97: 528535.
  • 185
    Vitetta ES, Fulton RJ, May RD, et al. Redesigning nature's poisons to create anti-tumor reagents. Science 1987; 238: 10981104.
  • 186
    Gadina M, Newton DL, Rybak SM, et al. Humanized immunotoxins. Ther Immunol 1994; 1: 5964.
  • 187
    Amlot PL, Stone MJ, Cunningham D, et al. A phase I study of an anti-CD22-deglycosylated ricin A chain immunotoxin in the treatment of B-cell lymphomas resistant to conventional therapy. Blood 1993; 82: 26242633.
  • 188
    Sausville EA, Headlee D, Stetler-Stevenson M, et al. Continuous infusion of the anti-CD22 immunotoxin IgG-RFB4-SMPT-dgA in patients with B-cell lymphoma: a phase I study. Blood 1995; 85: 34573465.
  • 189
    Stone MJ, Sausville EA, Fay JW, et al. A phase I study of bolus versus continuous infusion of the anti-CD19 immunotoxin, IgG-HD37-dgA, in patients with B-cell lymphoma. Blood 1996; 88: 11881197.
  • 190
    Smallshaw JE, Ghetie V, Rizo J, et al. Genetic engineering of an immunotoxin to eliminate vascular leak in mice. Nat Biotechnol 2003; 21: 387391.
  • 191
    Kreitman RJ, Squires DR, Stetler-Stevenson M, et al. Phase I trial of recombinant immunotoxin RFB4(dsFv)-PE38 (BL22) in patients with B-cell malignancies. J Clin Oncol 2005; 23: 67196729.
  • 192
    Posey JA, Khazaeli MB, Bookman MA, et al. A Phase I trial of the single-chain immunotoxin SGN-10 (BR96 sFv-PE-40) in patients with advanced solid tumors. Clin Cancer Res 2002; 8: 30923099.
  • 193
    Hellstrom I, Garrigues HJ, Garrigues U, Hellstrom KE. Highly tumor-reactive, internalizing, mouse monoclonal antibodies to Le(y)-related surface antigens. Cancer Res 1990; 50: 21832190.
  • 194
    Wittes RE. Cancer weapons, out of reach. The Washington Post. June 28, 2004.
  • 195
    Saltz LB, Lenz H, Hochster HS, et al. Randomized phase II trial of cetuximab/bevacizumab/irinotecan (CBI) versus cetuximab/bevacizumab (CB) in irinotecan-refractory colorectal cancer. J Clin Oncol 2005; 23: 248s.