Jennifer N. Hanson Shepherd, Sara T. Parker, Robert F. Shepherd, Martha U. Gillette, Jennifer A. Lewis and Ralph G. Nuzzo 3D Microperiodic Hydrogel Scaffolds for Robust Neuronal Cultures Advanced Functional Materials 21
3D microperiodic hydrogel scaffolds with varied architecture are patterned by direct-write assembly and investigated as culture platforms for primary hippocampal neurons. Neuronal cells thrive on these scaffolds, forming differentiated, intricately branched networks. Confocal laser scanning microscopy reveals that both cell distribution and extent of neuronal process alignment depend upon scaffold architecture.
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