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Indong Jun, Seok Joo Kim, Ji-Hye Lee, Young Jun Lee, Young Min Shin, Eunpyo Choi, Kyung Min Park, Jungyul Park, Ki Dong Park and Heungsoo Shin Transfer Printing of Cell Layers with an Anisotropic Extracellular Matrix Assembly using Cell-Interactive and Thermosensitive Hydrogels Advanced Functional Materials 22

Article first published online: 13 JUN 2012 | DOI: 10.1002/adfm.201200667

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A simple method to harvest cell layers with anisotropic extracellular matrix (ECM) assembly is presented using cell-interactive and thermoresponsive hydrogels with anisotropic groove patterns. When the temperature is reduced from 37 °C to 4 °C, the hydrogel expands its volume, while the cell layer can be detached and transferred onto new substrate without disruption of the cell–cell junction.

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