Mohammad Abdul Kadir, Su Jeong Kim, Eun-Ju Ha, Hong Y. Cho, Bong-Soo Kim, Donghyeuk Choi, Sun-Gu Lee, Bog G. Kim, Sang-Wook Kim and Hyun-jong Paik Encapsulation of Nanoparticles Using Nitrilotriacetic Acid End-Functionalized Polystyrenes and Their Application for the Separation of Proteins Advanced Functional Materials 22
Article first published online: 8 JUN 2012 | DOI: 10.1002/adfm.201200849
A simple method is developed for encapsulation of nanoparticles (NPs) for aqueous dispersion and selective separation of His-tagged proteins using nitrilotriacetic acid end-functionalized polystyrenes (NTA-PS). The complexation of Ni2+ with NTA on the surface of the nanocarrier containing magnetic NPs enables selective and smart separation of His-tagged proteins through NTA-Ni/His interaction from a multicomponent solution including a cell lysate using a magnet.
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