Eric C. Spivey, Eric T. Ritschdorff, Jodi L. Connell, Christopher A. McLennon, Christine E. Schmidt and Jason B. Shear Multiphoton Lithography of Unconstrained Three-Dimensional Protein Microstructures Advanced Functional Materials 23
Multiphoton lithography using a solid, protein-based reagent creates microstructures free from previous requirements for surface attachment. This approach is used to fabricate a broad range of microscale objects using several proteins. Free-floating 3D protein stars are confined with bacteria within microscopic chambers, providing a means to probe cellular activity using objects of defined shape. This method provides a tool for investigators to define and characterize cellular microenvironments.
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