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Qian Yu, Leah M. Johnson and Gabriel P. López Nanopatterned Polymer Brushes for Triggered Detachment of Anchorage-Dependent Cells Advanced Functional Materials 24

Version of Record online: 13 MAR 2014 | DOI: 10.1002/adfm.201304274

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Thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) brushes are nanopatterned via interferometric lithography and surface-initiated polymerization to support mammalian cell harvesting. Above the lower critical solution temperature (LCST), collapsed PNIPAAm brushes expose extracellular matrix proteins on the underlying substrate to support cellular attachment. As the temperature decreases below the LCST, swollen, extended PNIPAAm nanopatterned brushes readily release attached cells.

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