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Patrick L. Benitez, Jeffrey A. Sweet, Helen Fink, Krishna P. Chennazhi, Shantikumar V. Nair, Annika Enejder and Sarah C. Heilshorn Sequence-Specific Crosslinking of Electrospun, Elastin-Like Protein Preserves Bioactivity and Native-Like Mechanics Advanced Healthcare Materials 2

Version of Record online: 28 SEP 2012 | DOI: 10.1002/adhm.201200115

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A nanoscale mimic of the extracellular matrix is electrospun from a highly tunable family of elastin-like proteins. A sequence-specific, two-step crosslinking procedure is developed to preserve the nanofiber morphology, elastin-like mechanics, and specific bioactivity. Rodent marrow stromal cells show sequence-specific adhesion on the matrices, which are imaged using label-free coherent anti-Stokes Raman scattering (CARS) microscopy.

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