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Xiaohui Wu and Shanfeng Wang Biomimetic Calcium Carbonate Concentric Microgrooves with Tunable Widths for Promoting MC3T3-E1 Cell Functions Advanced Healthcare Materials 2

Version of Record online: 18 SEP 2012 | DOI: 10.1002/adhm.201200205

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Biomimetic, self-assembled calcium carbonate (CaCO3) concentric microgrooves with groove widths of 5.0 and 10 μm are fabricated by simply controlling incubation temperature. Mouse MC3T3-E1 cells are cultured on flat and microgrooved substrates of CaCO3 and their adhesion, spreading, proliferation, alkaline phosphatase activity, and calcium content are remarkably enhanced by the microgrooves, in particular, the narrower ones.

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