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Mohammad A. Alamein, Qin Liu, Sebastien Stephens, Stuart Skabo, Frauke Warnke, Robert Bourke, Peter Heiner and Patrick H. Warnke Nanospiderwebs: Artificial 3D Extracellular Matrix from Nanofibers by Novel Clinical Grade Electrospinning for Stem Cell Delivery Advanced Healthcare Materials 2

Version of Record online: 1 NOV 2012 | DOI: 10.1002/adhm.201200287

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A novel electrospinning process for engineering three-dimensional nanofi-brous extracellular matrixes (NFECMs) is initiated using a NanoSpider machine. Under clinical grade goodmanufacturing-practice-like conditions, several NF-ECMs are designed in a controlled manner to yield highly uniform fibers with sub-micrometer dimensions, favourable biomechanical properties and intrinsic cytobiocompatility. This permits both the proliferation and differentiation of human mesenchymal stem cells when provided appropriate biological cues.

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