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Bhushan P. Mahadik, Tobias D. Wheeler, Luke J. Skertich, Paul J. A. Kenis and Brendan A. C. Harley Microfluidic Generation of Gradient Hydrogels to Modulate Hematopoietic Stem Cell Culture Environment Advanced Healthcare Materials 3

Version of Record online: 29 AUG 2013 | DOI: 10.1002/adhm.201300263

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A microfluidic mixing platform is employed to generate 3D hydrogels containing stable, overlapping gradients of cell and matrix signals inspired by hematopoietic stem cell niches within the bone marrow. Primary hematopoietic stem and progenitor cells remain viable during mixing and culture within the bone marrow biochip platform. Analytical methods at multiple scales are described to examine hematopoietic cell bioactivity.

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