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Carole Cerclier, Aurélie Guyomard-Lack, Céline Moreau, Fabrice Cousin, Nadège Beury, Estelle Bonnin, Bruno Jean and Bernard Cathala Coloured Semi-reflective Thin Films for Biomass-hydrolyzing Enzyme Detection Advanced Materials 23

Article first published online: 25 JUL 2011 | DOI: 10.1002/adma.201101971

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A new enzymatic activity detection assay based on colour change of the semi-reflective films is presented. The method is based on the preparation of multilayered thin films of controlled thickness obtained by sequential deposition of cellulose nanocrystals and xyloglucan. The hydrolysis of the films leads to a decrease in layer thickness that enables to detect enzyme activity, to the naked eye, from the resulting colour changes in a span of few minutes. The method allows direct, fast, highly sensitive, and easy-to-use characterization of enzymatic activities.

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