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James C. Culver, Joseph C. Hoffmann, Ross A. Poché, John H. Slater, Jennifer L. West and Mary E. Dickinson Three-Dimensional Biomimetic Patterning in Hydrogels to Guide Cellular Organization Advanced Materials 24

Article first published online: 30 MAR 2012 | DOI: 10.1002/adma.201200395

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An image-guided micropatterning method is demonstrated for generating biomimetic hydrogel scaffolds with two-photon laser scanning photolithography. This process utilizes computational methods to directly translate three-dimensional cytoarchitectural features from labeled tissues into material structures. We use this method to pattern hydrogels that guide cellular organization by structurally and biochemically recapitulating complex vascular niche microenvironments with high pattern fidelity at the microscale.

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