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Christopher Schirwitz, Felix F. Loeffler, Thomas Felgenhauer, Volker Stadler, Alexander Nesterov-Mueller, Reiner Dahint, Frank Breitling and F. Ralf Bischoff Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes Advanced Materials 25

Version of Record online: 14 JAN 2013 | DOI: 10.1002/adma.201203853

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A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm2 is demonstrated.

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