Benjamin Richter, Thomas Pauloehrl, Johannes Kaschke, Dagmar Fichtner, Joachim Fischer, Alexandra M. Greiner, Doris Wedlich, Martin Wegener, Guillaume Delaittre, Christopher Barner-Kowollik and Martin Bastmeyer Three-Dimensional Microscaffolds Exhibiting Spatially Resolved Surface Chemistry Advanced Materials 25
Article first published online: 4 SEP 2013 | DOI: 10.1002/adma.201302678
Spatial control over the surface chemistry of 3D organic–inorganic hybrid microscaffolds is achieved by a two-photon-triggered cycloaddition. Following a coating step with photoactivatable dienes via silanization, surface irradiation with a femtosecond-pulsed laser in the presence of functional dienophiles enables a site-selective alteration of the surface chemistry. Bioconjugation with fluorescent protein tags is employed to reveal the 3D molecular patterns.
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