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Anton Glieder, Roland Weis, Wolfgang Skranc, Peter Poechlauer, Ingrid Dreveny, Sandra Majer, Marcel Wubbolts, Helmut Schwab and Karl Gruber Comprehensive Step-by-Step Engineering of an (R)-Hydroxynitrile Lyase for Large-Scale Asymmetric Synthesis Angewandte Chemie International Edition 42

Version of Record online: 29 SEP 2003 | DOI: 10.1002/anie.200352141

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A custom-made enzyme: The gene and protein sequence of a new (R)-hydroxynitrile lyase from Prunus amygdalus was cloned and engineered for recombinant production on a large scale. The enzyme is stable and active at low pH values. A rationally designed active-site mutant now permits the rapid and stereoselective synthesis of sterically hindered cyanohydrins such as ortho-chloromandelonitrile (see picture).

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