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Takeshi Serizawa, Miyuki Yamaguchi and Mitsuru Akashi Time-Controlled Desorption of Ultrathin Polymer Films Triggered by Enzymatic Degradation Angewandte Chemie International Edition 42

Version of Record online: 10 MAR 2003 | DOI: 10.1002/anie.200390293

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Degrading films: Surface-component-dependent and time-controlled degradation of a layer-by-layer (LbL) assembly comprising DNA and a cationic synthetic polymer is demonstrated through enzymatic hydrolysis by DNase I (see picture). The negatively charged enzyme is preserved on the cationic polymer surface in an inactive state, and changes in the concentrations of the essential Mg2+ and Ca2+ ions are used to control the activity of the enzyme, and thus control the desorption of the assembly.

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