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Muhammad Awais, Moritoshi Sato, Xianfen Lee and Yoshio Umezawa A Fluorescent Indicator To Visualize Activities of the Androgen Receptor Ligands in Single Living Cells Angewandte Chemie International Edition 45

Article first published online: 23 MAR 2006 | DOI: 10.1002/anie.200503185

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Seeing the light: An androgen promotes interactions between the androgen-receptor ligand-binding domain (AR LBD) and a coactivator protein. This results in an increase in the fluorescence resonant energy transfer (FRET) from cyan fluorescent protein (CFP) to yellow fluorescent protein (YFP). The indicator distinguishes ligands of different potencies for the AR.

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