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Mike Heilemann, Sebastian van de Linde, Mark Schüttpelz, Robert Kasper, Britta Seefeldt, Anindita Mukherjee, Philip Tinnefeld and Markus Sauer Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes Angewandte Chemie International Edition 47

Article first published online: 22 JUL 2008 | DOI: 10.1002/anie.200802376

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Eagle eyes: dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule.

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