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Zunyi Yang, Fei Chen, Stephen G. Chamberlin and Steven A. Benner Expanded Genetic Alphabets in the Polymerase Chain Reaction Angewandte Chemie International Edition 49

Version of Record online: 27 NOV 2009 | DOI: 10.1002/anie.200905173

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Cleaning up polymerase chain reactions: DNA polymerases are found that copy two additional nucleotide letters (Z and P) in an expanded DNA alphabet to support six-letter polymerase chain reactions (PCR). Incorporated into external primers in a threefold multiplexed PCR, primers containing Z and P gave much cleaner results than standard multiplexed PCR.

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