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Tilman Plass, Sigrid Milles, Christine Koehler, Priv.-Doz. Dr. Carsten Schultz and Dr. Edward A. Lemke Genetically Encoded Copper-Free Click Chemistry Angewandte Chemie International Edition 50

Article first published online: 23 MAR 2011 | DOI: 10.1002/anie.201008178

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A bright click of life: Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site-specifically in proteins allowing efficient labeling with fluorogenic azide-bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme).

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