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Tianqing Zheng, Dr. Hao Jiang, Dr. Marilyn Gros, Dr. David Soriano del Amo, Subha Sundaram, Prof. Grégoire Lauvau, Prof. Florence Marlow, Dr. Yi Liu, Prof. Pamela Stanley and Prof. Peng Wu Tracking N-Acetyllactosamine on Cell-Surface Glycans In Vivo Angewandte Chemie International Edition 50

Version of Record online: 29 MAR 2011 | DOI: 10.1002/anie.201100265

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In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

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