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Domenik Liße, Verena Wilkens, Dr. Changjiang You, Prof. Dr. Karin Busch and Prof. Dr. Jacob Piehler Selective Targeting of Fluorescent Nanoparticles to Proteins Inside Live Cells Angewandte Chemie International Edition 50

Version of Record online: 22 AUG 2011 | DOI: 10.1002/anie.201101499

Thumbnail image of graphical abstract

Tracking single proteins in cells: An optimized substrate based on a click reaction was designed for specific, irreversible targeting of nanoparticles to proteins fused to an engineered haloalkane dehalogenase (HaloTag) in the cytoplasm of life cells. Thus, proteins in the membrane of mitochondria could be tracked over extended times and with nanometer resolution (see picture).

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