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Prof. Bernard S. Munge, Amy L. Coffey, Jaimee M. Doucette, Brian K. Somba, Ruchika Malhotra, Dr. Vyomesh Patel, Dr. J. Silvio Gutkind and Prof. James F. Rusling Nanostructured Immunosensor for Attomolar Detection of Cancer Biomarker Interleukin-8 Using Massively Labeled Superparamagnetic Particles Angewandte Chemie International Edition 50

Article first published online: 30 JUN 2011 | DOI: 10.1002/anie.201102941

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A sensitive issue: Superparamagnetic particle–antibody conjugates enable detection of interleukin-8 (IL-8) in serum at a level of 1 fg mL−1 (ca. 100 aM). Ultrahigh sensitivity is facilitated by a nanostructured sensor platform coupled with secondary antibody–magnetic bead–horseradish peroxidase conjugate (Ab2–MB–HRP) with about 500 000 HRP labels (see picture).

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