Dr. Ghyslain Budin, Dr. Katherine S. Yang, Dr. Thomas Reiner and Prof. Ralph Weissleder Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification Angewandte Chemie International Edition 50
Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins.
Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf