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Dr. Mark R. Karver, Prof. Ralph Weissleder and Dr. Scott A. Hilderbrand Bioorthogonal Reaction Pairs Enable Simultaneous, Selective, Multi-Target Imaging Angewandte Chemie International Edition 51

Version of Record online: 12 DEC 2011 | DOI: 10.1002/anie.201104389

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Mutually orthogonal tetrazine–trans-cyclooctene and azide–cyclooctyne cycloaddition reactions were used simultaneously for the bioorthogonal labeling of two different live cell populations in the same culture (see scheme). These small-molecule probes show good chemical reactivity and can be readily incorporated into biological systems.

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