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Nathalie Ollivier, Dr. Jérôme Vicogne, Aurélie Vallin, Hervé Drobecq, Rémi Desmet, Ouafâa El Mahdi, Bérénice Leclercq, Dr. Gautier Goormachtigh, Dr. Véronique Fafeur and Dr. Oleg Melnyk A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis Angewandte Chemie International Edition 51

Version of Record online: 16 NOV 2011 | DOI: 10.1002/anie.201105837

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Three in one: Native chemical ligation (NCL) and bis(2-sulfanylethyl)amido (SEA) ligation allow the one-pot assembly of three peptide segments in the N-to-C direction. The SEA group (see picture, blue) is switched off by intramolecular disulfide bond formation during NCL. Then, a phosphine switches it on to trigger the second SEA ligation step. The K1 domain of the hepatocyte growth factor was synthesized and found to be biologically active.

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