Anmol Grover, Dr. Brigitte F. Schmidt, Dr. Russell D. Salter, Dr. Simon C. Watkins, Dr. Alan S. Waggoner and Dr. Marcel P. Bruchez Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis Angewandte Chemie International Edition 51
Traffic cam: A tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β2-adrenergic receptors (see scheme). It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).
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