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Dr. Masayuki Endo, Koichi Tatsumi, Kosuke Terushima, Yousuke Katsuda, Kumi Hidaka, Prof. Yoshie Harada and Prof. Hiroshi Sugiyama Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure Angewandte Chemie International Edition 51

Version of Record online: 29 JUL 2012 | DOI: 10.1002/anie.201201890

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Snapshots of transcription: Movement of a single molecule of T7 RNA polymerase (RNAP) along a double-stranded DNA (dsDNA) template attached to a DNA origami platform (green, see scheme) was recorded using high-speed AFM. A one kilobase template dsDNA containing the T7 promoter was used to record AFM images of transcription of the template dsDNA by RNAP and the resulting biotinylated RNA product was detected by streptavidin-labeling.

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