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Dr. Ryan M. Schweller, Jan Zimak, Dr. Dzifa Y. Duose, Dr. Amina A. Qutub, Prof. Walter N. Hittelman and Dr. Michael R. Diehl Multiplexed In Situ Immunofluorescence Using Dynamic DNA Complexes Angewandte Chemie International Edition 51

Version of Record online: 15 AUG 2012 | DOI: 10.1002/anie.201204304

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DNA-based erasers: The designed strand-displacement reactions between dynamic DNA probes (carrying fluorescent dyes (★) and quencher domains (•)) and DNA–antibody conjugates generate detachable immunofluoresence-reporting complexes that can be assembled (first image) and disassembled (second image) reversibly within fixed cells. With this system, different protein targets can be imaged sequentially within the same cells (third image).

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