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Dr. Amritraj Patra, Michael Paolillo, Dr. Klaus Charisse, Dr. Muthiah Manoharan, Prof. Eriks Rozners and Prof. Martin Egli 2′-Fluoro RNA Shows Increased Watson–Crick H-Bonding Strength and Stacking Relative to RNA: Evidence from NMR and Thermodynamic Data Angewandte Chemie International Edition 51

Version of Record online: 10 OCT 2012 | DOI: 10.1002/anie.201204946

Thumbnail image of graphical abstract

The way it is: Incorporation of 2′-deoxy-2′-fluororibonucleotides (2′-F RNA) into RNA oligomers leads to a marked increase in duplex stability. Using NMR spectroscopy (see picture) with native (red circles) and 2′-F modified (black squares) RNA duplexes, and UV melting and osmotic stress experiments the higher stability of 2′-F RNA relative to RNA is shown to be the result of both increased Watson–Crick H-bonding strength and favorable base stacking.

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